Enhancers and Promoters
Springer-Verlag New York Inc.
978-1-0716-1599-7 (ISBN)
Authoritative and cutting-edge, Enhancers and Promoters: Methods and Protocols is a useful guide for future experiments.
Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com
A New Toolbox to Analyze Enhancer-Promoter Functions.- Global Run-on Sequencing (GRO-Seq).- Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET-Seq).- Low Quantity single strand CAGE (LQ-ssCAGE) Maps Regulatory Enhancers and Promoters.- Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase-Seq.- Measuring Chromatin Accessibility: ATAC-Seq.- High-resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles.- Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes.- Low Input Targeted Chromatin Capture (low-T2C).- Proximity Ligation-assisted ChIP-Seq (PLAC-Seq).- Analysis of Enhancer-promoter Interactions using CAGE and RADICL-Seq technologies.- Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to identify open chromatin interactions.- Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C).- Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-Translational Modification (PTM) Combinatorics At Distinct Genomic Regions.- Using ChIP-SICAP to Identify Proteins That Co-Localize in Chromatin.- Genome-wide profiling of protein-DNA Interactions with Chromatin Endogenous Cleavage And High-Throughput Sequencing (ChEC-Seq).- Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators.- Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors.- Simultaneous Tagmentation-based detection of ChIP/ATAC Signal with Bisulfite Sequencing.- Low Input Whole-Genome Bisulfite Sequencing.
| Erscheinungsdatum | 18.08.2022 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 2351 |
| Zusatzinfo | 57 Illustrations, color; 12 Illustrations, black and white; XI, 376 p. 69 illus., 57 illus. in color. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Schlagworte | eRNAs • Histone • inflammation • Myc locus • T-Cells |
| ISBN-10 | 1-0716-1599-8 / 1071615998 |
| ISBN-13 | 978-1-0716-1599-7 / 9781071615997 |
| Zustand | Neuware |
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