Signal Transduction Protocols

Robert C. Dickson, Michael D. Mendenhall (Autoren)

Buch | Hardcover

340 Seiten

2004 | 2nd Revised edition
Humana Press Inc. (Verlag)
978-1-58829-245-2 (ISBN)

Infos

Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated the first edition with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class.

Inhalt

Making Protein Immunoprecipitates Elaine A. Elion and Yunmei Wang Signal Transduction Inhibitors in Cellular Function Maofu Fu, Chenguang Wang, Xueping Zhang, and Richard G. Pestell Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets Yukihito Kabuyama, Kirsi K. Polvinen, Katheryn A. Resing, and Natalie G. Ahn A High-Throughput Mammalian Cell-Based Transient Transfection Assay Daniel J. Noonan, Kenneth Henry, and Michelle L. Twaroski Determining Protein Half-Lives Pengbo Zhou Assaying Protein Kinase Activity Jan Brabek and Steven K. Hanks Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach Francesca Zappacosta, Michael J. Huddleston, and Roland S. Annan Studies of Calmodulin-Dependent Regulation Paul C. Brandt and Thomas C. Vanaman Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation Hogune Im, Jeffrey A. Grass, Kirby D. Johnson, Meghan E. Boyer, Jing Wu, and Emery H. Bresnick Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation Laurent Kuras Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins Douglas A. Andres Nucleocytoplasmic Glycosylation, O-GlcNAc: Identification and Site Mapping Natasha Elizabeth Zachara, Win Den Cheung, and Gerald Warren Hart Techniques in Protein Methylation Jaeho Lee, Donghang Cheng, and Mark T. Bedford Assaying Lipid Phosphate Phosphatase Activities Gil-Soo Han and George M. Carman Assaying Phosphoinositide Phosphatases Gregory S. Taylor and Jack E. Dixon Assaying Phospholipase A2 Activity Christina C. Leslie and Michael H. Gelb Measurement and Immunofluorescence of Cellular Phosphoinositides Hiroko Hama, Javad Torabinejad, Glenn D. Prestwich, and Daryll B. DeWald Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer Sandrine Evellin, Marco Mongillo, Anna Terrin, Valentina Lissandron, and Manuela Zaccolo In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET Chitra Subramanian, Yao Xu, Carl Hirschie Johnson, and Albrecht G. von Arnim Revealing Protein Dynamics by Photobleaching Techniques Frank van Drogen and Matthias Peter Assaying Cytochrome c Translocation During Apoptosis Nigel J. Waterhouse, Rohan Steel, Ruth Kluck, and Joseph A. Trapani Index

Produkt-Details

• Erscheint lt. Verlag: 18.6.2004
• Reihe/Serie: Methods in Molecular Biology ; 284
• Zusatzinfo: 1
• Verlagsort: Totowa, NJ
• Sprache: englisch
• Maße: 234 x 156 mm
• Gewicht: 653 g
• Einbandart: gebunden
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• ISBN-10: 1-58829-245-2 / 1588292452
• ISBN-13: 978-1-58829-245-2 / 9781588292452
• Zustand: Neuware