T-Cell Development
Springer-Verlag New York Inc.
978-1-0716-2739-6 (ISBN)
Authoritative and cutting-edge, T-Cell Development: Methods and Protocols, Second Edition aims to be a useful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development.
A beginner’s guide to T cell development.- Revelations in thymic epithelial cell biology and heterogeneity from single cell RNA sequencing and lineage tracing methodologies.- Early development of innate lymphoid cells.- Development of gd T cells, Soldiers on the Front Lines of Immune Battles.- Principles of Advanced Flow Cytometry: A Practical Guide.- Genetic strategies to study T cell development.- A simplified approach to evaluating T cell development by flow cytometry.- Purification of thymocyte and T cell subsets.- Generation of bone marrow chimeras.- Generation of murine T cell effector populations in vitro.- Large-scale isolation of mouse thymic epithelial cells.- Generation of retrogenic mice to investigate T cell developmentPurification of bone marrow precursors to T cells and ILCs.- Studying T cell Development in Neonatal and Adult Thymic Slices.- Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines.- Methods for Study of Mouse T Cell Receptor a and b Gene Rearrangements.- Ultrasound guided intra-thymic cell injection.- Fetal thymic organ culture and negative selection.- In Vitro Analysis of Thymocyte Signaling.- Identification and purification of human T cell precursors.- In vitro model systems to study human T cell development.- Zebrafish: A Tractable Model for Analysis of T cell Development.
Erscheinungsdatum | 17.11.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2580 |
Zusatzinfo | 41 Illustrations, color; 35 Illustrations, black and white; XI, 384 p. 76 illus., 41 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
ISBN-10 | 1-0716-2739-2 / 1071627392 |
ISBN-13 | 978-1-0716-2739-6 / 9781071627396 |
Zustand | Neuware |
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