Pseudogenes

Functions and Protocols

Buch | Softcover

384 Seiten

2022 | 2nd ed. 2021
Springer-Verlag New York Inc.
978-1-0716-1505-8 (ISBN)

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This second edition explores techniques involving pseudogenic DNA, RNA, and proteins. Chapters describe how to identify pseudogenes and how to study the functions and alterations of pseudogenic RNA and proteins, both in vitro, and in vivo. Written in the highly successful Methods in Molecular Biology series format, methodological chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting while avoiding known pitfalls.

Authoritative and cutting-edge, Pseudogenes: Functions and Protocols, Second Edition aims to be a practical guide to researches, useful to help further their study in this field.

Pseudogenes: Four Decades of Discovery.- Methods to Identify and Study the Evolution of Pseudogenes using a Phylogenetic Approach.- Computational Methods For Pseudogene Annotation Based on Sequence Homology.- A Methodology to Study pseudogenized lincRNAs.- GENCODE Pseudogenes.- Comprehensive Detection of Pseudogenes Transcribed by Readthrough.- Mathematical modeling of ceRNA-based interactions.- Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation.- Pseudogenes as Competitive Endogenous RNAs: Testing miRNA Dependency.- An Overview of the Computational Models Dealing with the Regulatory ceRNA Mechanism and ceRNA Deregulation in Cancer.- Studying the Oncosuppressive Functions of PTENP1 as a ceRNA.- Methods to Study Protein-Binding to Pseudogene Transcript.- Chromatin Regulation at Parental Gene Promoters by Pseudogene Sense lncRNAs.- Pseudogenes: A Novel Source of Trans Acting Antisense RNAs.- Methods to Study Translated Pseudogenes: Recombinant Expression and Complementation, Targeted Proteomics and RNA Profiling.- A Restriction Endonuclease-Based Assay to distinguish NANOGP8 Retrogene from Parental NANOG.- CRISPR/Cas Technologies Applied to Pseudogenes.- Strategies to Study the Functions of Pseudogenes in Mouse Models of Cancer.- Pseudogene Profiling For Cancer Subtype Classification.- Pseudogenes as Biomarkers and Therapeutic Targets in Human Cancers.- Methods for the Detection of Circulating Pseudogenes and their use as Cancer Biomarker.- Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era.

Erscheinungsdatum	30.06.2022
Reihe/Serie	Methods in Molecular Biology ; 2324
Zusatzinfo	78 Illustrations, color; XIII, 384 p. 78 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
Schlagworte	Evolutionary Measure dN/dS • Homologous recombination • PCR • RNA-Seq • sequence homology
ISBN-10	1-0716-1505-X / 107161505X
ISBN-13	978-1-0716-1505-8 / 9781071615058
Zustand	Neuware