Endocannabinoid Signaling
Springer-Verlag New York Inc.
978-1-0716-2727-3 (ISBN)
Comprehensive and cutting-edge, Endocannabinoid Signaling: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.
Need for Methods to Investigate Endocannabinoid Signaling.- Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-like Lipids in Biological Tissues.- Measuring the Content of Endocannabinoid-like Compounds in Biological Fluids: A Critical Overview of Sample Preparation Methodologies.- LC-MS/MS Analysis of AEA and 2-AG.- Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices.- Endocannabinoid-Binding Receptors as Drug Targets.- Assay of CB1 Receptor Binding.- The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells.- Fluorescence-based Assay for TRPV1 Channels.- DNA-Protein-Interaction (DPI)-ELISA Assay for PPAR-γ Receptor Binding.- Scintillation Proximity Assay (SPA)-Based Radioligand Binding for PPARα, PPARγ and PPARδ Receptors.- A Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPARα andPPARγ.- The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells.- Assay of GTPγS Binding in Autoradiography.- Cellular Assay to Study β-Arrestin Recruitment by the Cannabinoid Receptors 1 and 2.- Endocannabinoid Metabolism and Transport as Drug Targets.- Assay of NAT Activity.- Radiometric Assay of NAPE-PLD activity.- A Fluorescence-based NAPE-PLD Activity Assay.- Radiometric Assay of FAAH Activity.- Fluorimetric Assay of FAAH activity.- Assay of NAAA Activity.- Assay of DAGLα/β Activity.- Assay of Monoacylglycerol Lipase Activity.- Radiometric Assay of ABHD2 Activity.- Oxygenation of Anandamide by Lipoxygenases.- Assay of Endocannabinoid Oxidation by Cytochrome P450.- Assays of Endocannabinoid Uptake.- Assessing Gene Expression of the Endocannabinoid System Components by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction.- Bioinformatics of the Endocannabinoid System: Study of DNA methylation at Rat Cnr1 gene promoter.- DNA Methylation Analysis of Cnr1 Gene Promoter.- Western Blotting of the Endocannabinoid System.- Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells.- Assessing CB1 Expression in the Brain by Immunohistochemical Methods: Light, Confocal and Electron Microscopy.- Measuring Endocannabinoid System Interaction with Biomembranes.- STORM Super-Resolution Imaging of CB1 Receptors in Tissue Preparations.- Visualization of Endocannabinoids in the Cell.- Electrophysiology of Endocannabinoid Signalling.- Machine Learning and Computational Chemistry for the Endocannabinoid System.- A Virtual Reverse Screening Approach to Target Type 2 Cannabinoid Receptor.
| Erscheinungsdatum | 17.10.2022 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 2576 |
| Zusatzinfo | 65 Illustrations, color; 73 Illustrations, black and white; XVII, 509 p. 138 illus., 65 illus. in color. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie |
| Naturwissenschaften ► Biologie ► Allgemeines / Lexika | |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Schlagworte | Cannabinoids • drug design • Drug targets • Endogenous counterpart of THC • membrane and nuclear receptors • signaling pathways |
| ISBN-10 | 1-0716-2727-9 / 1071627279 |
| ISBN-13 | 978-1-0716-2727-3 / 9781071627273 |
| Zustand | Neuware |
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