ELISA

Advances in Research and Applications

Buch | Hardcover

242 Seiten

2022
Nova Science Publishers Inc (Verlag)
978-1-68507-500-2 (ISBN)

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In September 1971, Swiss scientists Eva Engvall and Peter Perlmann published a communication in the journal Immunochemistry on enzyme-linked immunosorbent assay (ELISA) as a quantitative assay of immunoglobulin G. They have modified radioimmunoassay (RIA) by replacing the radioactive iodine with enzymes and used in determining the levels of IgG in rabbit serum. Fifty years after, ELISA is still the most commonly used technique in measuring peptides, proteins, antibodies, antigens, and hormones in various samples. This plate-based assay is a very versatile laboratory technique and offers many advantages to laboratory scientists and researchers such as generating quick results, simple protocols, high sensitivity, being easily automated and being convenient to perform. Its application in the diagnosis of different infectious diseases has shown the unquestionable importance of this technique even in the modern world. In this book, the usefulness of ELISA in the control of different parasitic diseases including schistosomiasis, babesiosis, and malaria will be discussed. There is also a chapter featuring an extensive discussion on the use of ELISA in functional food development and regulation. And finally, the last two chapters will present advanced techniques inspired by ELISA including a rapid immunoassay for detecting paralytic shellfish poisoning toxins and the enzyme-linked aptamer assay. May this book provide significant information regarding the current advances and applications of ELISA and possibly inspire its use in another 50 years.

Preface; Updates in the Diagnostic Use of ELISA for Schistosomiasis; Use of an ELISA to Assess the Immunogenicity and Protective Potential of a Babesia Bigemina RAP-1, GP45, and 12D3 Recombinant Protein Cocktail in Cattle; ELISA Test Using Babesia Sp. Recombinant Antigens for Epidemiological Diagnosis of Bovine Babesiosis in Mexico; ELISA: A Sero-Surveillance Tool in Malaria Elimination; Applications of Enzyme-Linked Immunosorbent Assays in Functional Food Development and Regulation; A Refined Rapid Antibody-Based Immunoassay Improved Sensitivity of Paralytic Shellfish Poisoning Toxins Detection; Aptamers: The Chemical Antibodies, Their Emergence, and Application to Enzyme-Linked Immunosorbent Assay; Index.

Erscheinungsdatum	17.02.2022
Verlagsort	New York
Sprache	englisch
Maße	152 x 229 mm
Gewicht	462 g
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Anamnese / Körperliche Untersuchung
ISBN-10	1-68507-500-2 / 1685075002
ISBN-13	978-1-68507-500-2 / 9781685075002
Zustand	Neuware