Mouse Genetics

Methods and Protocols

Shree Ram Singh, Robert M. Hoffman, Amit Singh (Herausgeber)

Buch | Softcover

217 Seiten

2022 | 2nd ed. 2021
Springer-Verlag New York Inc.
978-1-0716-1010-7 (ISBN)

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This fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.

Generation of Mouse Model (KI & CKO) via Easi-CRISPR.- Analysis of Gene Expression Using lacZ Reporter Mouse Lines.- Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.- Mouse Models for Studying Hippocampal Adult Neural Stem Cell Biology.- Retina as a Model to Study In Vivo Transmission of α-Synuclein in the A53T Mouse Model of Parkinson’s Disease.- Promoting Pro-Endocrine Differentiation and Graft Maturation Following Surgical Resection of the Mouse Pancreas.- Color-Coded Imaging of Cancer and Stromal Cells Interaction in the Pancreatic Cancer Tumor Microenvironment (TME).- Generating Ins2+/-/miR-133aTg Mice to Model miRNA-Driven Cardioprotection of Diabetic Hearts in Humans.- Generating a Podocyte-Specific FcRn Knockout Mouse.- Mouse Models of Colitis-Associated Colon Cancer.- Colon Cancer Tumor Model Base on Colonoscopy Injection.- Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse.- Gene Inactivation in Adult Long Term-Hematopoietic Stem Cells Using Inducible Mouse Models.- Hematological Humanization of Immune Deficient Mice.- Antisense Oligonucleotide Treatment in a Humanized Mouse Model of Duchenne Muscular Dystrophy and Highly Sensitive Detection of Dystrophin Using Western Blotting.

Erscheinungsdatum	09.03.2022
Reihe/Serie	Methods in Molecular Biology ; 2224
Zusatzinfo	41 Illustrations, color; 6 Illustrations, black and white; XI, 217 p. 47 illus., 41 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie
	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	CRISPR/Cas9 • Developmental Biology • Human disease models • Mouse Models • Transgenic mice
ISBN-10	1-0716-1010-4 / 1071610104
ISBN-13	978-1-0716-1010-7 / 9781071610107
Zustand	Neuware