Ewing Sarcoma
Springer-Verlag New York Inc.
978-1-0716-1022-0 (ISBN)
Cutting-edge and clearly written, Ewing sarcoma: Methods and Protocols aims to provide methods to further the study of Ewing sarcoma and bring researchers one step forward in the quest to fight this devastating disease.
Germline Variation and Somatic Alterations in Ewing Sarcoma.- Western Blot Analysis in Ewing Sarcoma.- Tissue Preservation and FFPE Samples: Optimized Nucleic Acids Isolation in Ewing Sarcoma.- Liquid Biopsies in Ewing Sarcoma.- (Immuno-)histological Analysis of Ewing Sarcoma.- Molecular Approaches to Diagnosis In Ewing Sarcoma: Fluorescence in situ hybridization (FISH).- Molecular Approaches to Diagnosis in Ewing Sarcoma: RT-PCR.- Molecular Approaches to Diagnosis in Ewing Sarcoma: Targeted RNA Sequencing.- Ewing-specific (re)expression Models.- Analysis of Regulatory Sequences by Dual-luciferase Reporter Assays in Ewing Sarcoma.- Proliferation Assessment by Trypan Blue Exclusion in Ewing Sarcoma.- Drug Screening by Resazurin Colorimetry in Ewing Sarcoma.- Analysis of Migration and Invasion in Ewing Sarcoma.- Genetically Engineered Mouse Model in Ewing Sarcoma.- Tumor Growth Analysis of Ewing Sarcoma CellLines Using Subcutaneous Xenografts in Mice.- Metastasis Assessment in Ewing Sarcoma Using Orthotopic Xenografts.- Orthotopic Implants in Mice.- Ewing Sarcoma PDX Models.- Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma.- Main Repositories and Databases used in Ewing Sarcoma Research.- Chromatin Immunoprecipitation Followed by Next-Generation Sequencing (ChIP-seq) Analysis in Ewing Sarcoma.- Epigenetic Analysis in Ewing Sarcoma.- Systems Biology Analysis for Ewing Sarcoma.
Erscheinungsdatum | 29.12.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2226 |
Zusatzinfo | 50 Illustrations, color; 7 Illustrations, black and white; XI, 337 p. 57 illus., 50 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie |
Medizin / Pharmazie ► Studium | |
Schlagworte | Epigenetic alterations • fish • qRT-PCR • RNA and DNA sequencing • Zebrafish |
ISBN-10 | 1-0716-1022-8 / 1071610228 |
ISBN-13 | 978-1-0716-1022-0 / 9781071610220 |
Zustand | Neuware |
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