Mixed-Mode Chromatography

Principles, Methods, and Applications

Qian-Hong Wan (Autor)

Buch | Hardcover

482 Seiten

2021 | 1st ed. 2021
Springer Verlag, Singapore
978-981-16-5484-8 (ISBN)

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The book is about the technology and application of Mixed-mode chromatography (MMC). Unlike conventional single-mode HPLC, which resolves the analytes primarily based on their ionic or hydrophobic properties, MMC employs multifunctional stationary phases to exploit at least two modes of interactions (i.e., ionic and hydrophobic) with the analytes and as such often provides resolution that far exceeds that observed with a single-mode process. Over the past two decades, MMC has developed into an important analytical and purification tool in a number of applications in pharmaceutical and biotechnology industries. The technique has been used widely for the analyses of nucleic acids, amino acids, peptides, proteins, glycoproteins, carbohydrates, antibiotics, vaccines, and other products. The purpose of this book is to present a comprehensive survey of mixed-mode chromatography and is intended as a reference guide for graduate students and experienced scientists in pharmaceutical and biotechnology disciplines wishing to gain a deep understanding of this continuously evolving technology.

Qian-Hong Wan is a professor in the Pharmaceutical Analysis department, School of Pharmaceutical Science and Technology, Tianjin University. He obtained his Ph.D. from Edinburgh University in 1993 and completed his two post-doctoral training at University of Amsterdam and Nottingham University. His research is focused on modern separation and analysis technology.

Chapter 1.Historical Perspectives.- Chapter 2.Theoretical Background.- Chapter 3.Modes of Chromatographic Separation.- Chapter 4.Chromatographic Matrices .- Chapter 5.Ligand Design.- Chapter 6.Retention Mechanisms.- Chapter 7.Stationary Phases.- Chapter 8.Mobile Phases and Elution.- Chapter 9.Method Development.- Chapter 10.Applications in Biopharmaceutical Processing. .

Erscheinungsdatum	06.12.2021
Zusatzinfo	65 Illustrations, color; 151 Illustrations, black and white; XIII, 482 p. 216 illus., 65 illus. in color.
Verlagsort	Singapore
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin
	Medizin / Pharmazie ► Pharmazie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Chemie ► Analytische Chemie
	Naturwissenschaften ► Chemie ► Technische Chemie
	Technik
Schlagworte	Biological Samples • Chromatography • Mixed-mode separations • Pharmaceutical Analysis • Retention process
ISBN-10	981-16-5484-0 / 9811654840
ISBN-13	978-981-16-5484-8 / 9789811654848
Zustand	Neuware