Single Molecule Microscopy in Neurobiology

Nobuhiko Yamamoto, Yasushi Okada (Herausgeber)

Buch | Softcover

331 Seiten

2021 | 1st ed. 2020
Springer-Verlag New York Inc.
978-1-0716-0534-9 (ISBN)

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This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field.

Single Molecule Imaging of Intracellular Transport in Neurons and Non-Neuronal Cells: From Microscope Optics to Sample Preparations.- In Vivo Single-Molecule Tracking of Voltage-Gated Calcium Channels with Split-Fluorescent Proteins in CRISPR-Engineered C. elegans.- Nanocores and Liquid Droplets: Single-Molecule Microscopy of Neuronal Stress Granule Components.- Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of an Activity-Dependent Transcription Factor.- Single-Molecule Imaging of Recycling Synaptic Vesicles in Live Neurons.- Approaching Protein‐Protein Interactions in Membranes using Single Particle Tracking and Packing Coefficient Analysis.- Synaptic Function and Neuropathological Disease Revealed by Quantum-Dot Single Particle Tracking.- Multipolarization Darkfield Imaging of Single Endosomes in Microfluidic Neuronal Culture for Simultaneous Orientation and Displacement Tracking.- Practical Guidelines for Two-Color SMLM of Synaptic Proteins in Cultured Neurons.- Single-Molecule Localization Microscopy Propelled by Small Organic Fluorophores with Blinking Properties.- Highly-Biocompatible Super-Resolution Imaging: SPoD-OnSPAN.- Nanoscale Molecular Imaging of Presynaptic Active Zone Proteins in Cultured Hippocampal Neurons.- Three Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons using Selective Plane Illumination.- A Protocol for Single Molecule Translation Imaging in Xenopus Retinal Ganglion Cells.- Investigating Molecular Diffusion Inside Small Neuronal Compartments with Two-Photon Fluorescence Correlation Spectroscopy.

Erscheinungsdatum	07.05.2021
Reihe/Serie	Neuromethods ; 154
Zusatzinfo	73 Illustrations, color; 21 Illustrations, black and white; XIII, 331 p. 94 illus., 73 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
	Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
ISBN-10	1-0716-0534-8 / 1071605348
ISBN-13	978-1-0716-0534-9 / 9781071605349
Zustand	Neuware