Mycobacteria Protocols
Springer-Verlag New York Inc.
978-1-0716-1459-4 (ISBN)
Authoritative and up-to-date, Mycobacteria Protocols, Fourth Edition serves as an ideal guide for those starting out with their mycobacteria research and also for those who have worked with it for decades.
Culturing Mycobacteria.- DNA Isolation from Mycobacteria.- Extraction and Separation of Mycobacterial Proteins.- Lipid and Lipoarabinomannan Isolation and Characterization.- Purification of Hibernating and Active C- Ribosomes from Zinc-Starved Mycobacteria.- Macrophage Infection Models for Mycobacterium tuberculosis.- Novel Single-Cell and High Throughput Microscopy Techniques to Monitor Dictyostelium discoideum-Mycobacterium marinum Infection Dynamics.- Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy.- Measuring Efflux and Permeability in Mycobacteria.- Drug Susceptibility Screening Using In Vitro Models of Hypoxic Non-Replicating Persistent Mycobacteria.- Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells.- Electroporation of Mycobacteria.- Gene Switching and Essentiality Testing.- Oligo-Mediated Recombineering and Its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis.- Using Proteolytic Hypomorphs to Detect Small Molecule Mechanism of Action.- CRISPR Interference (CRISPRi) for Targeted Gene Silencing in Mycobacteria.- Exploiting Fluorescent Proteins to Understand Mycobacterium tuberculosis Biology.- Metabolic Labeling of Live Mycobacteria with Trehalose-Based Probes.- Identification and Characterization of Mycobacterial Species Using Whole Genome Sequences.- Whole Genome Sequencing of Mycobacterium tuberculosis Directly from Sputum Samples.- Experimental and Computational Workflow for RNA Sequencing in Mycobacterium tuberculosis: From Total RNA to Differentially Expressed Genes.- RNA Sequencing for Transcript 5′-End Mapping in Mycobacteria.- Methods for Proteomic Analyses of Mycobacteria.- Targeted Lipidomics of Mycobacterial Lipids and Glycolipids.- Metabolomics of Mycobacterium tuberculosis.- Determining Minimum Inhibitory Concentrations in Liquid Cultures or on Solid Medium.- A Multi-Stress Model for High Throughput Screening Against Non-Replicating Mycobacterium tuberculosis.- Antimicrobial Susceptibility Testing for Mycobacterium sps in High Throughput Format.- High-Content Analysis Monitoring Intracellular Trafficking and Replication of Mycobacterium tuberculosis Inside Host Cells.- Efficient Measurement of Drug Interactions with DiaMOND (Diagonal Measurement of N-Way Drug Interactions).- Drug Sensitivity Testing of Mycobacterium tuberculosis Growing in a Hollow Fiber Bioreactor.
Erscheinungsdatum | 16.07.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2314 |
Zusatzinfo | 80 Illustrations, color; 24 Illustrations, black and white; XV, 736 p. 104 illus., 80 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Pathologie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-0716-1459-2 / 1071614592 |
ISBN-13 | 978-1-0716-1459-4 / 9781071614594 |
Zustand | Neuware |
Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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