Regulatory B Cells
Springer-Verlag New York Inc.
978-1-0716-1236-1 (ISBN)
Authoritative and cutting-edge, Regulatory B Cells: Methods and Protocols, Second Edition aims to be useful to the scientific community and serve toclarify some unsolved aspects of Regulatory B Cells research.
Purification and characterization of murine MZ and T2-MZP cells.- Purification and immune-phenotyping of B-1 cells from body cavities of mice.- Purification and immunophenotypic characterization of murine plasma cells.- Purification of murine and human IL-10 producing B cells from different anatomical compartments.- Purification and immunophenotypic characterization of human CD19+CD24hiCD38hi and CD19+CD24hiCD27+ B cells.- Detection of IL-10 in murine B cells: in vitro and in vivo techniques.- Detection and quantification of transforming growth factor-β1 produced by murine B cells: pros and cons of different techniques.- IL-35 detection in B cells at the mRNA and protein level.- Characterization and activation of Fas Ligand producing mouse B cells and their killer exosomes.- Characterization and activity of TIM-1 and IL-10-reporter expressing regulatory B cells.- New method for the expansion of highly purified human regulatory granzyme B-expressing B cells.- Characterization of the cell surface phenotype and regulatory activity of B cell IgD low (BDL) regulatory B cells.- Use of Toll-like receptor (TLR) ligation to characterize human regulatory B cells subsets.- Use of co-cultures for the study of cellular interactions influencing B cell regulatory functions.- Use of inhibitory compounds to dissect the molecular pathways involved in regulatory B cell differentiation.- Methods to study the transcriptome of regulatory B cells.- Purification of murine IL-10+ B cells for analyses of biological functions and transcriptomics.- il-10 gene locus DNA methylation in regulatory B cells.- B cell commitment to IL-10 production: the Vert-X Il10egfp mouse.- Regulatory B cells in experimental mouse models of arthritis.- Regulatory and IgE+ B cells in allergic asthma.- Regulatory B cells in type 1 diabetes.- Analysis of regulatory B cells in experimental autoimmune uveitis.- Purification and immunophenotypic characterization of human CD24hiCD38hi and CD24hiCD27+ regulatory B cells in transplantation.
Erscheinungsdatum | 29.01.2021 |
---|---|
Reihe/Serie | Methods in Molecular Biology ; 2270 |
Zusatzinfo | 66 Illustrations, color; 24 Illustrations, black and white; XIV, 471 p. 90 illus., 66 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Studium ► Querschnittsbereiche ► Infektiologie / Immunologie |
ISBN-10 | 1-0716-1236-0 / 1071612360 |
ISBN-13 | 978-1-0716-1236-1 / 9781071612361 |
Zustand | Neuware |
Haben Sie eine Frage zum Produkt? |
aus dem Bereich