Experimental Cholestasis Research

Buch | Softcover

399 Seiten

2020 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9422-9 (ISBN)

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This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic andapplied researchers pursuing this vital area of research in pharmacology and toxicology.

Mechanisms of Drug-Induced Cholestasis.- LC-MS/MS Analysis of Bile Acids in In Vitro Samples.- Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis.- Vesicle-Based and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function.- The C-DILI™ Assay: An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity.- In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids.- Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy.- The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis.- Measuring Apoptosis and Necrosis in Cholestatic Liver Injury.- Necrosome Formation and Necroptosis in Experimental Cholestasis.- Proteomics in Primary Biliary Cholangitis.- Transcriptomics Analysis of Cholestatic Compounds In Vitro.- Systems Microscopy of Stress Response Pathways in Cholestasis Research.- Mouse Models for Diseases in the Cholangiocyte Lineage.- Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis.- 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis Research.- A Rotavirus-Induced Mouse Model to Study Biliary Atresia and Neonatal Cholestasis.- Zebrafish as a Model to Study Cholestatic Liver Diseases.- Set-Up and Use of HepaRG Cells in Cholestasis Research.- Organotypic 3D HepaRG Liver Model for Assessment of Drug-Induced Cholestasis.- Establishment of Sandwich Cultures of Primary Human Hepatocytes.- Detection of Drug-Induced Cholestasis Potential in Sandwich-Cultured Human Hepatocytes.- Ex Vivo Model in Cholestasis Research.- Patient-Derived Organoids from Human Bile: An In Vitro Method to Study Cholangiopathies.- Use of Biliary Organoids in Cholestasis Research.- In Silico Approaches to Predict Drug-Transporter Interaction Profiles: Data Mining, Model Generation, and Link to Cholestasis.

Erscheinungsdatum	07.07.2020
Reihe/Serie	Methods in Molecular Biology ; 1981
Zusatzinfo	60 Illustrations, color; 14 Illustrations, black and white; XIII, 399 p. 74 illus., 60 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizinische Fachgebiete ► Innere Medizin ► Hepatologie
	Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie
	Medizin / Pharmazie ► Pharmazie
Schlagworte	Cholestatic drug properties • Dili • drug-induced liver injury • Hepatocellular injury • Impaired bile secretion • Liver toxicology • Model organisms
ISBN-10	1-4939-9422-0 / 1493994220
ISBN-13	978-1-4939-9422-9 / 9781493994229
Zustand	Neuware