Citrus Tristeza Virus

Methods and Protocols

Antonino F. Catara, Moshe Bar-Joseph, Grazia Licciardello (Herausgeber)

Buch | Softcover

247 Seiten

2020 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9560-8 (ISBN)

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This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease.

A Short Note on Reflections and Publications on Citrus Tristeza Virus (CTV) Methodologies.- A Brief Historical Account of the Family Closteroviridae.- Phenotyping Biological Properties of CTV Isolates.- CTV Vectors and Interactions with the Virus and Host Plants.- Tissue Print and Squash Captures Real-Time RT-PCR Method for Direct Detection of Citrus Tristeza Virus (CTV) in Plant or Vector Tissues.- Detection of Citrus Tristeza Virus and Co-Infecting Viroids.- Assessment of Genetic Variability of Citrus Tristeza Virus by SSCP and CE-SSCP.- Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus Tristeza Virus.- Genotyping Citrus Tristeza Virus Isolates by Sequential Multiplex RT-PCR and Microarray Hybridization in a Lab-On-Chip Device.- Rapid and Sensitive Detection of Citrus Tristeza Virus Using Reverse Transcription-Loop-Mediated Isothermal Ampliﬁcation (RT-LAMP) Assay.- Amplification and Cloning of Large cDNA Fragments of the Citrus Tristeza Virus Genome.- Bioinformatic Tools and Genome Analysis of Citrus Tristeza Virus.- Analysis of Genotype Composition of Citrus Tristeza Virus Populations Using Illumina Miseq Technology.- Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction.- Proteomic Response of Host Plants to Citrus Tristeza Virus.- Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment.- Methods for Producing Transgenic Plants Resistant to CTV.

Erscheinungsdatum	09.07.2020
Reihe/Serie	Methods in Molecular Biology ; 2015
Zusatzinfo	36 Illustrations, color; 14 Illustrations, black and white; X, 247 p. 50 illus., 36 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Naturwissenschaften ► Biologie ► Botanik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Closteroviridae • Closterovirus • CTV • Deep sequencing • Plant pathologists • Plant virologists • RT-PCR
ISBN-10	1-4939-9560-X / 149399560X
ISBN-13	978-1-4939-9560-8 / 9781493995608
Zustand	Neuware