RNA Editing

Methods and Protocols

Ernesto Picardi, Graziano Pesole (Herausgeber)

Buch | Hardcover

352 Seiten

2020 | 1st ed. 2021
Springer-Verlag New York Inc.
978-1-0716-0786-2 (ISBN)

Artikel merken

This volume provides an overview about main RNA editing mechanisms, focusing on their functions in physiological as well as pathological conditions. Chapters guide readers through state- of-the art methodologies to investigate RNA editing through wet and dry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, RNA Editing: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Substitutional RNA Editing in Plant Organelles.- Computational Detection of Plant RNA Editing Events.- Discovering RNA Editing Events in Fungi.- C-to-U RNA Editing: From Computational Detection to Experimental Validation.- Live Cell Quantification of APOBEC1-mediated RNA Editing: A Comparison of RNA Editing Assays.- Adenosine to Inosine RNA Editing Enzyme ADAR and microRNAs.- Quantitative Analysis of Adenosine-to-Inosine RNA Editing.- Discovering A-to-I RNA Editing Through Chemical Methods.- ALU A-to-I RNA Editing - Millions of Sites and Many Open Questions.- RNA Editing in Human and Mouse Tissues.- Bioinformatics Resources for RNA Editing.- High-throughput Sequencing to Detect DNA-RNA Changes.- Detection of A-to-I Hyper-Edited RNA Sequences.- Proteome Diversification by RNA Editing.- MicroRNA Editing Detection and Function: A Combined in silico and Experimental Approach for the Identification and Validation of Putative Oncogenic Targets.- RNA Editing in Interferonopathies.- The Role of RNA Editing in the Immune Response.- RNA Editing in Neurological and Neurodegenerative Disorders.- New Frontiers for Site-directed RNA Editing - Harnessing Endogenous ADARs.

Erscheinungsdatum	07.08.2020
Reihe/Serie	Methods in Molecular Biology ; 2181
Zusatzinfo	43 Illustrations, color; 4 Illustrations, black and white; XV, 352 p. 47 illus., 43 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
ISBN-10	1-0716-0786-3 / 1071607863
ISBN-13	978-1-0716-0786-2 / 9781071607862
Zustand	Neuware