Homologous Recombination
Springer-Verlag New York Inc.
978-1-0716-0643-8 (ISBN)
Cutting-edge and thorough, Homologous Recombination: Methods and Protocols is a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. It is a useful tool for scientists working on the field of genome integrity, as well as those working in cancer biology and DNA repair.
Detection of DNA Double Strand Breaks by γ-H2AX Immunodetection.- END-seq: An Unbiased, High-Resolution and Genome-Wide Approach to Map DNA Double Strand Breaks and Resection in Human Cells.- Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting.- Analysis of DNA Double-Strand Break Ends Resection and Single-Strand Annealing in S. pombe.- Quantifying DNA End Resection in Human Cells.- Genetic and Molecular approaches to Study Chromosomal Breakage at Secondary Structure-Forming Repeats.- Biochemical analysis of D-Loop Extension and DNA Strand Displacement Synthesis.- DNA Strand Exchange to Monitor Human RAD51-Mediated Strand Invasion and Pairing.- Monitoring Homologous Recombination Activity in Human Cells.- Inter-Homolog Homologous Recombination in Mouse Embryonic Stem Cells.- Branch Migration Activity of Rad54 Protein.- Holliday Junction Resolution.- Identification and Analysis of Different Types of UFBs.- Intrachromosomal Recombination in Yeast.- Genome-Wide Analysis of Mitotic Recombination in Budding Yeast.- Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis.- DNA Double-Strand Break Induced Gene Amplification in Yeast.- Measuring Chromosome Pairing during Homologous Recombination in Yeast.- Cytological Monitoring of Meiotic Crossovers in Spermatocytes and Oocytes.- Detection of DSBs in C. elegans Meiosis.- Methods to Map Meiotic Recombination Proteins in Saccharomyces cerevisiae.- Investigation of Break-Induced Replication in Yeast.- Measurement of Homologous Recombination at Stalled Mammalian Replication Forks.- Super Resolution Imaging of Homologous Recombination Repair at Collapsed Replication Forks.- The Analysis of Recombination-Dependent Processing of Blocked Replication Forks by Bi-Dimensional Gel Electrophoresis.- The Sister Chromatid Exchange Assay in Human Cells.- Analysis of Recombination at Yeast Telomeres.- Gel Electrophoresis Analysis of rDNA Instability in Saccharomyces cerevisiae.- Analyzing Homologous Recombination at a Genome Wide Level.- CRISPR/Cas9 Induced Breaks in Heterochromatin, Visualized by Immunofluorescence.- In Vivo Binding of Recombination Proteins of Non-DSB DNA Lesions to Replication Forks.- Live Cell Imaging of Nuclear Actin Filaments and Heterochromatic Repair foci in Drosophila and Mouse Cells.- In Vitro Characterization of Sumoylation of HR Proteins.- High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products.- Fluorescence Microscopy for Analysis of Relocalization of Structure-Specific Endonucleases.- Physical and Genetic Assays for the Study of DNA Joint Molecules Metabolism and Multi-Invasion-Induced Rearrangements in S. cerevisiae.
| Erscheinungsdatum | 28.08.2020 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 2153 |
| Zusatzinfo | 91 Illustrations, color; 35 Illustrations, black and white; XV, 557 p. 126 illus., 91 illus. in color. With online files/update. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
| Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
| ISBN-10 | 1-0716-0643-3 / 1071606433 |
| ISBN-13 | 978-1-0716-0643-8 / 9781071606438 |
| Zustand | Neuware |
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