Toxoplasma gondii
Humana Press Inc. (Verlag)
978-1-4939-9856-2 (ISBN)
Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.
What a Difference 30 years Makes! A Perspective on Changes in research Methodologies used to Study Toxoplasma gondii.- ToxoDB: Functional Genomics Resource for Toxoplasma and Related Organisms.- Isolation and Genotyping of Toxoplasma gondii Strains.- Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of their Sporozoites for Downstream Manipulation.- Assay for Monitoring Toxoplasma gondii Infectivity in the Laboratory Mouse.- PCR Screening of Toxoplasma gondii Single Clones Directly from 96-Well Plantes without DNA Purification.- CRISPR/Cas9-Mediated Generation of Tetracycline Repressor-Based Inducible Knock-Down in Toxoplasma gondii.- Assessing Rhyptry Secretion in T. gondii.- High-Throughput Measurement of Microneme Secretion in Toxoplasma gondii.- Plate-Based Quantification of Stimulated Toxoplasma egress.- Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondii.- Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging.- Experimental Approaches for Examining Apicoplast Biology.- Measuring Solute Transport in Toxoplasma gondii Parasites.- Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In Vivo.- Three-Dimensional Reconstruction of Toxoplasma-Neuron Interactions In Situ.- Model Systems for Studying Mechanisms of Ocular Toxoplasmosis.- Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii.- Assays to Evaluate Toxoplasma-Macrophage Interactions.- Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells.- Image-based Quantitation of Host Cell-Toxoplasma gondii Interplay using HRMan; A Host Response to Microbe Analysis Pipeline.- Metabolomic Analysis of Toxoplasma gondii Tachyzoites.- Label-Based Mass Spectrometry Approaches for Robust Quantification of the Phospho- and Total Proteome in Toxoplasma gondii.
Erscheinungsdatum | 16.12.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 2071 |
Zusatzinfo | 63 Illustrations, color; 14 Illustrations, black and white; XI, 470 p. 77 illus., 63 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Studium ► Querschnittsbereiche ► Infektiologie / Immunologie |
Schlagworte | Apical organelles • bradyzoites • Host cell Egress • mouse model • Tissue culture |
ISBN-10 | 1-4939-9856-0 / 1493998560 |
ISBN-13 | 978-1-4939-9856-2 / 9781493998562 |
Zustand | Neuware |
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