Reporter Gene Imaging

Methods and Protocols

Buch | Softcover

221 Seiten

2019 | Softcover reprint of the original 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-9306-2 (ISBN)

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This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.

Dual Modality Imaging of Promoter Activity as a Surrogate for Gene Expression and Function.- Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging.- Optical Bioluminescence Protocol for Imaging Mice.- Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase.- Reporter Based BRET Sensors for Measuring Biological Functions In Vivo.- Fluorescence Imaging of Mycobacterial Infection in Live Mice Using Fluorescent Protein-Expressing Strains.- In vivo Bacterial Imaging using Bioluminescence.- Production of GFP and Luciferase Expressing Reporter Macrophages for In Vivo Bioluminescence Imaging.- Hypoxia-induced Reporter Genes with Different Half-lives.- Visualization of Immune System Reconstitution by Bioluminescent Imaging.- Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo.- Ex vivo radiolabeling and in vivo PET imaging of T cells expressing nuclear reporter genes.- Imaging T Cell Dynamics and Function byPET using Human Nuclear Reporter Genes.- Positron Emission Tomography Imaging of Tumor Apoptosis with a Caspase-sensitive Nano-Aggregation Tracer [18F]C-SNAT.- Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes.- Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models.

Erscheinungsdatum	15.07.2019
Reihe/Serie	Methods in Molecular Biology ; 1790
Zusatzinfo	42 Illustrations, color; 11 Illustrations, black and white; XI, 221 p. 53 illus., 42 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizinische Fachgebiete ► Radiologie / Bildgebende Verfahren ► Radiologie
Schlagworte	bioluminescent imaging • Fluorescent • PET radiotracers • positron emission tomography (PET) • radioisotopic • thymidine kinase (TK)
ISBN-10	1-4939-9306-2 / 1493993062
ISBN-13	978-1-4939-9306-2 / 9781493993062
Zustand	Neuware