Reporter Gene Assays

Methods and Protocols

Robert Damoiseaux, Samuel Hasson (Herausgeber)

Buch | Softcover

234 Seiten

2019 | Softcover reprint of the original 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-9268-3 (ISBN)

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This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs. Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interestedin learning more about reporter genes.

Genome-Edited Cell Lines for High-Throughput Screening.- High-Throughput Firefly Luciferase Reporter Assays.- Using the 2A Protein Co-Expression System: Multi-Cistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins.- Developing Mammalian Cellular Clock Models Using Firefly Luciferase Reporter.- High-Throughput Screening Method to Identify Alternative Splicing Regulators.- High-Throughput Screening of a Luciferase Reporter of Gene Silencing on the Inactive X Chromosome.- Making It All Work: Functional Genomics and Reporter Gene Assays.- Reporter Gene Assays Using Transfectable Functional Genomics Libraries.- Reporter Gene Assays Using Viral Functional Genomics Libraries.- Using YFP as a Reporter of Gene Expression in the Green Alga Chlamysomonas reinhardtii.- Gene Expression Analysis by Arylsulfatase Assays in the Green Algo Chlamydomonas reinhardtii.- Endogenous Locus Reporter Assays.- High Content Reporter Assays.- Data Mining and Computational Modeling of High-Throughput Screening Datasets.- Intravital Imaging by Two-Photon Excitation Microscopy of Human Melanoma Cells in the Mouse Ear Skin.

Erscheinungsdatum	25.07.2019
Reihe/Serie	Methods in Molecular Biology ; 1755
Zusatzinfo	42 Illustrations, color; 11 Illustrations, black and white; XII, 234 p. 53 illus., 42 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
Schlagworte	Cellular functions • gene expression patterns • genes • high-throughput drug screening • NFkB activation in immune cells • pathogenic protein expression
ISBN-10	1-4939-9268-6 / 1493992686
ISBN-13	978-1-4939-9268-3 / 9781493992683
Zustand	Neuware