Handbook of Molecular and Cellular Methods in Biology and Medicine
Crc Press Inc (Verlag)
978-0-8493-2511-3 (ISBN)
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Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method.
This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies.
The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!
Isolation and Purification of DNA
Isolation and Purification of RNA
Extraction and Purification of Proteins/Enzymes
Preparation of Nucleic Acid Probes
Electrophoresis, Blotting, and Hybridization
cDNA Libraries
Genomic DNA Libraries
DNA Sequencing
PCR Techniques and Applications
DNA Fingerprinting
DNA Mutagenesis In Vitro
Inhibition of Gene Expression by Antisense DNA and RNA
DNA Footprinting and Gel Retardation Assay
Translation of mRNA(s) In Vitro and Analysis of Proteins by Gel Electrophoresis
Gene Transfer and Expression in Animals
Gene Transfer and Expression in Plants
Plant Cell Culture
Plant Tissue Culture
Microscopy: LM, SEM, TEM, Confocal and Applications
Bioseparation Techniques and Their Applications
Preparation of Monoclonal and Polyclonal Antibodies against Specific Protein(s)
| Erscheint lt. Verlag | 15.5.1995 |
|---|---|
| Zusatzinfo | 22 Halftones, black and white; 284 Tables, black and white |
| Verlagsort | Bosa Roca |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Gewicht | 880 g |
| Einbandart | Paperback |
| Themenwelt | Medizin / Pharmazie ► Studium ► 1. Studienabschnitt (Vorklinik) |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Humanbiologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 0-8493-2511-0 / 0849325110 |
| ISBN-13 | 978-0-8493-2511-3 / 9780849325113 |
| Zustand | Neuware |
| Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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