Glioblastoma

Methods and Protocols

Buch | Softcover

241 Seiten

2019 | Softcover reprint of the original 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-8536-4 (ISBN)

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This volume details cutting-edge protocols on the characterization of the genome, epigenome, proteome, metabolome and single-cell transcriptome of tumors and tumor-derived cultures. Chapters focus on subpopulations of cells with stem-like properties, laser capture microdissection, and modeling human glioma with human embryonic stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glioblastoma: Methods and Protocols aims to support researchers seeking new and refined protocols to better decrypt this tumor.

Whole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma.- Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina BeadArrays.- Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens.- Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens.- Isolation of GBM Stem Cells with Flow Cytometry.- Lentiviral Transduction of Primary Human Glioblastoma Cultures.- Selective Targeting of CD133-expressing Glioblastoma Stem Cells using Lentiviral Vectors.- Intracellular pH Measurements in Glioblastoma Cells using the pH-sensitive Dye BCECF.- Induction and Assessment of Hypoxia in Glioblastoma Cells in vitro.- Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography-tandem Mass Spectrometry.- Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS).- Single-Cell RNA Sequencing of Glioblastoma Cells.- Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures.- Orthotopic Patient-derived Glioblastoma Xenografts in Mice.- Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice.- Evaluation of Vascularity, Blood Perfusion, and Oxygen Tension in Tumor Xenografts with Fluorescent Microscopy.- Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection.- Flow Cytometric Identification of Tumor-infiltrating Lymphocytes from Glioblastoma.- Modeling Glioma with Human Embryonic Stem Cell-derived Neural Lineages.

Erscheinungsdatum	18.03.2019
Reihe/Serie	Methods in Molecular Biology ; 1741
Zusatzinfo	43 Illustrations, color; 9 Illustrations, black and white; XI, 241 p. 52 illus., 43 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
Themenwelt	Medizin / Pharmazie ► Studium
Schlagworte	Embryonic Stem Cells • GBM cancer stem cells • Neuro-oncology • Tumor • xenografts
ISBN-10	1-4939-8536-1 / 1493985361
ISBN-13	978-1-4939-8536-4 / 9781493985364
Zustand	Neuware