G Protein-Coupled Receptor Signaling
Humana Press Inc. (Verlag)
978-1-4939-9120-4 (ISBN)
Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening.
Chapter 4 is available open access under a CC BY 4.0 license via link.springer.com.
Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics.- Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs.- Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies.- A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties.- Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions.- Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands.- Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs.- Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology.- Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches.- Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays.- Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions.- Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters.- Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays.- Measurement of β-Arrestin Recruitment at GPCRs Using the Tango Assay.- Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry.- Monitoring the Aggregation of GPCRs by Fluorescence Microscopy.- Combining RNAi and Immunofluorescence Approaches to Investigate Post-Endocytic Sorting of GPCRs into Multivesicular Bodies.- Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy.- Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy.- Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug.- Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice.- Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G Protein-Coupled Receptors in Rodents.- Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs.
| Erscheinungsdatum | 22.03.2019 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1947 |
| Zusatzinfo | 71 Illustrations, color; 16 Illustrations, black and white; XV, 406 p. 87 illus., 71 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Biologie ► Humanbiologie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zoologie | |
| Schlagworte | Brain physiology • Developmental Biology • drug development • GPCR drugs • Molecular Pharmacology • Transmembrane protein families |
| ISBN-10 | 1-4939-9120-5 / 1493991205 |
| ISBN-13 | 978-1-4939-9120-4 / 9781493991204 |
| Zustand | Neuware |
| Haben Sie eine Frage zum Produkt? |
aus dem Bereich
