Germinal Centers

Methods and Protocols

Buch | Softcover

338 Seiten

2018 | Softcover reprint of the original 1st ed. 2017
Humana Press Inc. (Verlag)
978-1-4939-8383-4 (ISBN)

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This volume provides key methods and protocols from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Germinal Centers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Analysis of the Germinal Center Reaction in Tissue Sections.- Detecting Gene Expression in Lymphoid Microenvironments by Laser Microdissection and Quantitative RT-PCR.- Tracking Plasma Cell Differentiation in Living Mice with 2-Photon Microscopy.- Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation.- Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-photon Photoconversion.- Intravital Microscopy of T-B Cell Interactions in Germinal Centers.- Identifying Follicular Regulatory T cells by Confocal Microscopy.- Cytokine Expression by T follicular Helper Cells.- Follicular Dendritic Cell Isolation and Loading of Immune Complexes.- Isolation and Characterization of Mouse and Human Follicular Dendritic Cells.- In-vitro-induced Germinal Center B Cell Culture System.- CRISPR/Cas9 Mediated In Vitro Mutagenesis in GC-like B Cells.- Germinal Center Formation with Retrovirally-transduced B Cells for Determining the Role of Specific Molecules In Vivo.- Characterization of the B Cell Transcriptome by RNA-Binding Proteins using iCLIP.- Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity.- Somatic Hypermutation and Affinity Maturation Analysis Using the 4-hydroxy-3-nitrophenyl-acetyl (NP) System.- Targeting Gene Function in Germinal Center B Cells: A Practical Approach.- Development of Mouse Model Systems of Germinal Centre Lymphomas.- The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas.- Determining the Origin of Human Germinal Center B Cell-derived Malignancies.- Tracking B-cell Repertoires and Clonal Histories in Normal and Malignant Lymphocytes.- How to Simulate a Germinal Centre.

Erscheinungsdatum	30.10.2018
Reihe/Serie	Methods in Molecular Biology ; 1623
Zusatzinfo	49 Illustrations, color; 15 Illustrations, black and white; XI, 338 p. 64 illus., 49 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
ISBN-10	1-4939-8383-0 / 1493983830
ISBN-13	978-1-4939-8383-4 / 9781493983834
Zustand	Neuware