Legionella

Methods and Protocols

Carmen Buchrieser, Hubert Hilbi (Herausgeber)

Buch | Hardcover

476 Seiten

2019 | 2nd ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9047-4 (ISBN)

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The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.

Analysis of Iron Requirements and Siderophore Production.- The Pathometabolism of Legionella Studied by Isotopologue Profiling.- Quantification of Viable but Non-culturable Cells of Legionella pneumophila.- Selection of Legionella Virulence-related Traits by Environmental Protozoa.- Migration of Acanthamoeba castellanii through Legionella Biofilms.- Scar-free Genome Editing in Legionella pneumophila.- Querying Legionella Genomes using Transposition-sequencing.- Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq.- Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae.- Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium.- Sorting of Phagocytic Cells Infected with Legionella pneumophila.- Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment.- Mitochondrial Dynamics and Activity in Legionella-Infected Cells.- Perturbation of Legionella Cell Infection by RNA Interference.- Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila.- In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography.- Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins.- Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila.- Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification.- Inflammasome Activation in Legionella-infected Macrophages.- Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila.- The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence.- Dictyostelium Host Response to Legionella Infection: Strategies and Assays.- 6.1.3 The Caenorhabditis elegans model of Legionella infection.- The Mouse as a Model for Pulmonary Legionella Infection.- Targeted Metagenomics of Microbial Diversity in Free-living Amoebae and Water Samples.- Analyses of the Pulmonary Microbiome Composition of Legionella Pneumophila-Infected Patients.- Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry.

Erscheinungsdatum	19.02.2019
Reihe/Serie	Methods in Molecular Biology ; 1921
Zusatzinfo	50 Illustrations, color; 24 Illustrations, black and white; XXVI, 476 p. 74 illus., 50 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizinische Fachgebiete ► Innere Medizin ► Pneumologie
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
Schlagworte	Amobea • Biofilm • Imaging Flow Cytometry • L. pneumophila Infection • pneumophila
ISBN-10	1-4939-9047-0 / 1493990470
ISBN-13	978-1-4939-9047-4 / 9781493990474
Zustand	Neuware