Calcium-Binding Proteins of the EF-Hand Superfamily
Humana Press Inc. (Verlag)
978-1-4939-9029-0 (ISBN)
Authoritative and comprehensive, Calcium-Binding Proteins ofthe EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.
Using Data Mining to Explore Calmodulin Bibliography.- Deep Two-Photon Imaging In Vivo with a Red-Shifted Calcium Indicator.- High Resolution Calcium Imaging Method for Local Calcium Signaling.- Measurement of Contractility and Calcium Release in Cardiac Spheroids.- Simultaneous Recording of Subcellular Ca2+ Signals from the Cytosol and Sarco/Endoplasmatic Reticulum: Compartmentalized Dye Loading, Imaging, and Analysis.- The Use of Complementary Luminescent and Fluorescent Techniques for Imaging Ca2+ Signalling Events during the Early Development of Zebrafish (Danio rerio).- Cellular Ca2+-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements.- Designing Calcium Binding Proteins for Molecular MR Imaging.- Coordination to Divalent Cations by Calcium Binding Proteins.- Chaperoning against Amyloid Aggregation: Monitoring In Vitro and In Vivo.- Ca2+-Binding Proteins of theEF-Hand Superfamily: Diagnostic and Prognostic Biomarkers and Novel Therapeutic Targets.- Gene Transfer of Calcium Binding Proteins into Adult Cardiac Myocytes.- Expression and Purification of Calmodulin for NMR and Other Biophysical Applications.- The Use of Cre-loxP Inducible Mouse Models to Dissect the Specific Roles of Calcineurin Signalling in Myeloid Cells.- Calpain Purification through Calpastatin and Calcium: Strategy and Procedures.- Characterization of the EF-Hand Calcium-Binding Domains of Human Plastins.- Expression and Characterization of MICU2, a Ca2+ Sensor Protein.- S100 Proteins in the Innate Immune Response to Pathogens.- Targeting S100 Calcium-Binding Proteins with Small Molecule Inhibitors.- Monitoring Interactions between S100B and the Dopamine D2 Receptor Using NMR Spectroscopy.- Isolation and Characterization of S100 Protein-Protein Complexes.- The Multifaceted S100A4 Protein in Cancer and Inflammation.- Interaction of S100A6 with Target Proteins In Vitro and in Living Cells.- Preparation of the Oxidized and Reduced Forms of Psoriasin (S100A7).- Preparation and Iron Redox Speciation Study of the Fe(II)-Binding Antimicrobial Protein Calprotectin.- Structural Analysis of S100A8 Complex with Zinc and Calcium: A General Protocol for the Study of S100 Proteins in the Presence of Divalent Cations by X-Ray Crystallography.- Analysis of Ca2+-Dependent Weibel-Palade Body Tethering by Live Cell TIRF Microscopy: Involvement of a Munc13-4/S100A10/Annexin A2 Complex.- Analysis of S100A11 in DNA Damage Repair.- Fluorine-18 Labeling of S100 Proteins for Small Animal Positron Emission Tomography.- Reviewing the Crystal Structure of S100Z and Other Members of the S100 Family: Implications in Calcium-Regulated Quaternary Structure.- High Sensitive Quantitative Binding Assays Using a Nanoluciferase-Fused Probe for Analysis of ALG-2-Interacting Proteins.- Calcium-Induced Protein Folding in Calumenin and Calmodulin.- Measuring CalumeninImpact on ER-Calcium Depletion Using Transient Calumenin Overexpression and Silencing.- Secretagogin Purification and Quality Control Strategies for Biophysical and Cell Biological Studies.- Tryptophan Scanning Mutagenesis of EF-Hand Motifs.- Mapping Calcium-Sensitive Regions in GCAPs by Site-Specific Fluorescence Labeling.- Quantification of Human Swiprosin-1/EFhd2 Expression on Protein and RNA Level.- Three-Dimensional Reconstruction Imaging Method to Study the Function of EFHD2 in Invadopodia Formation.- Characterization of Calcium-Binding Proteins from Parasitic Worms.- High-Sensitivity Troponin Assays in Clinical Diagnostics of Acute Coronary Syndrome.- Targeted Mass Spectrometry of S100 Proteins.- Clinical Use of the Calcium-Binding S100B Protein, a Biomarker for Head Injury.- Serum S100B Levels in Melanoma.- The Ca2+-Binding S100B Protein: An Important Diagnostic and Prognostic Neurobiomarker in Pediatric Laboratory Medicine.- S100A7 in Psoriasis: Immunodetection andActivation by CRISPR.- S100A8/A9 in Myocardial Infarction.- Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults.- S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions.
| Erscheinungsdatum | 28.02.2019 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1929 |
| Zusatzinfo | 117 Illustrations, color; 58 Illustrations, black and white; XIX, 779 p. 175 illus., 117 illus. in color. With online files/update. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | Ca2+-signaling • calcium-binding • Personalized medicine • pharmacology • Therapeutic drug targets |
| ISBN-10 | 1-4939-9029-2 / 1493990292 |
| ISBN-13 | 978-1-4939-9029-0 / 9781493990290 |
| Zustand | Neuware |
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