Cell-free DNA as Diagnostic Markers

Methods and Protocols

Valentina Casadio, Samanta Salvi (Herausgeber)

Buch | Hardcover

224 Seiten

2018 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-8972-0 (ISBN)

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This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical activity, and urinary cell free DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cell-Free DNA as Diagnostic Markers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Cell Free DNA: Applications in Different Diseases.- Cell Free DNA: An Overview of Sample Types and Isolation Procedures.- Circulating Cell Free DNA and Cancer Therapy Monitoring: Methods and Potential.- Cell Free DNA in the Liquid Biopsy Context: Role and Differences between ctDNA and CTC Marker in Cancer Management.- Cell Free DNA Integrity: Applications.- Copy Number Variation Analysis on Cell Free Serum DNA.- Studying Copy Number Variations in Cell Free DNA: The Example of AR in Prostate Cancer.- dPCR Mutational Analyses in Cell-free DNA: A Comparison With Tissues.- From cfDNA to Sequencing: Workflows and Potentials.- Epigenetic Characterization of Cell Free DNA.- Quantitative Methylation-specific PCR: A Simple Method for Studying Epigenetic Modifications of Cell Free DNA.- Cell Free DNA in Pediatric Rhabdomyosarcoma: Potential and Challenges.- Bioinformatics Pipeline for Accurate Quantification of Fetal DNA Fraction in Maternal Plasma.- Circulating Cell-Free DNA in Physical Activities.- Urinary Cell Free DNA: Potential and Applications.- Urinary Cell Free DNA: Isolation, Quantification, Quality Assessment.

Erscheinungsdatum	07.02.2019
Reihe/Serie	Methods in Molecular Biology ; 1909
Zusatzinfo	27 Illustrations, color; 2 Illustrations, black and white; X, 224 p. 29 illus., 27 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
Schlagworte	Bioinformatics • Cancer • DNA integrity • Mutations analyses • Urinary cell free DNA
ISBN-10	1-4939-8972-3 / 1493989723
ISBN-13	978-1-4939-8972-0 / 9781493989720
Zustand	Neuware