Nicht aus der Schweiz? Besuchen Sie lehmanns.de
Systematic Exploration of Indolizine-Based Small Fluorescent Molecules - Youngjun Lee

Systematic Exploration of Indolizine-Based Small Fluorescent Molecules (eBook)

Synthesis, Analysis and Application

(Autor)

eBook Download: PDF
2018 | 1st ed. 2018
XXV, 187 Seiten
Springer Singapore (Verlag)
978-981-13-1645-6 (ISBN)
Systemvoraussetzungen
96,29 inkl. MwSt
(CHF 93,95)
Der eBook-Verkauf erfolgt durch die Lehmanns Media GmbH (Berlin) zum Preis in Euro inkl. MwSt.
  • Download sofort lieferbar
  • Zahlungsarten anzeigen
This thesis describes an in-depth study of an indolizine-based fluorophore, from understanding of its structure-photophysical property relationship to its application as a useful biological reporter.
Organic fluorophores have been extensively used in the field of molecular biology owing to their excellent photophysical property, suitable cell permeability, and synthetic flexibility. Understanding of the structure-photophysical property relationship of a given fluorophore often paves the road to the development of valuable molecular probes to visualize and transcribe biological networks. In this thesis, respective chapters deal with molecular design, organic synthesis, structure-property analysis, and quantum-mechanical interpretation of unexplored family of indolizine-based molecules. This systematic exploration has led to rational development of a new microalgae lipid droplet probe, colorful bioorthogonal fluorogenic probes, and a bright mitochondrial probe, working under live cell conditions.
Harnessing the optical properties of a given fluorophore has been an important topic for a couple of decades, both in industry and in academia. This thesis provides useful insights for the improvement and development of unique small fluorescent materials, or optical materials.


Dr. Youngjun Lee is a member of CRI Center for Chemical Proteomics, Department of Chemistry, Seoul National University, Korea. He earned Ph.D. degree in organic chemistry at SNU in February 2018.

?
This thesis describes an in-depth study of an indolizine-based fluorophore, from understanding of its structure-photophysical property relationship to its application as a useful biological reporter.Organic fluorophores have been extensively used in the field of molecular biology owing to their excellent photophysical property, suitable cell permeability, and synthetic flexibility. Understanding of the structure-photophysical property relationship of a given fluorophore often paves the road to the development of valuable molecular probes to visualize and transcribe biological networks. In this thesis, respective chapters deal with molecular design, organic synthesis, structure-property analysis, and quantum-mechanical interpretation of unexplored family of indolizine-based molecules. This systematic exploration has led to rational development of a new microalgae lipid droplet probe, colorful bioorthogonal fluorogenic probes, and a bright mitochondrial probe, working underlive cell conditions.Harnessing the optical properties of a given fluorophore has been an important topic for a couple of decades, both in industry and in academia. This thesis provides useful insights for the improvement and development of unique small fluorescent materials, or optical materials.

Dr. Youngjun Lee is a member of CRI Center for Chemical Proteomics, Department of Chemistry, Seoul National University, Korea. He earned Ph.D. degree in organic chemistry at SNU in February 2018. ​

AbstractTable of ContentsList of FiguresList of TablesList of SchemesList of AbbreviationsChapter 1. Introduction

1.1 Molecular Fluorescence and Photophysical Parameters

1.2 Organic Fluorophores

1.2.1 BODIPY

1.2.2 Coumarin

1.2.3 Cyanine

1.2.4 Fluorescein

1.2.5 Rhodamine

1.2.6 Seoul-Fluor

1.3 Aims and Scope of the Thesis

1.4 References

Chapter 2. A Comprehensive Studies of an Indolizine-Based Seoul-Fluor System

2.1 Structure and Quantum Yield Relationship Study of Seoul-Fluor

2.1.1 Introduction

2.1.2 Result and discussion

2.1.3 Conclusion

2.2 Optimization of SF-Based Lipid Droplet Bioprobes on the basis of Molecular Lipophilicity 2.2.1 Introduction

2.2.2 Result and discussion

2.2.3 Conclusion

2.3 An Overview of Seoul-Fluor System

2.4 References

2.5 Experimental Section

Chapter 3. Tetrazine-Containing Colorful Bioorthogonal Probes based on the Indolizine Core Skeleton

3.1 Introduction

3.2 Result and Discussion

3.2.1 Initial design and synthesis

3.2.2 Change in absorption property of SFTz01–05 upon TCO cycloaddition reaction 3.2.3 Change in fluorescence property of SFTz01–05 upon cycloaddition reaction with TCO 3.2.4 Reaction kinetics

3.2.5 Rational expansion of multicolor SFTzs based on a monochromophoric strategy 3.2.6 TD-DFT calculation

3.2.7 Direct comparison of mono- vs bichromophoric SFTzs with long emission wavelength

3.2.8 Bioapplication

3.3 Conclusion

3.4 References

3.5 Experimental Section

Chapter 4. Rational Development of Furoindolizine Core Skeleton Guided by Oscillator Strength

4.1 Introduction

4.2 Result and Discussion

4.2.1 Designing an unexplored fluorophore with high molar absorptivity 4.2.2 Library construction based on a novel furo[3,2-e]indolizine scaffold 4.2.3 Photophysical property analysis

4.2.4 Rational design of novel furoindolizine-based fluorescent compounds and bioapplication 4.3 Conclusion

4.4 References

4.5 Experimental Section

Erscheint lt. Verlag 16.8.2018
Reihe/Serie Springer Theses
Springer Theses
Zusatzinfo XXV, 187 p. 197 illus., 90 illus. in color.
Verlagsort Singapore
Sprache englisch
Themenwelt Medizin / Pharmazie Pflege
Medizin / Pharmazie Physiotherapie / Ergotherapie Orthopädie
Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
Naturwissenschaften Chemie Organische Chemie
Naturwissenschaften Physik / Astronomie Angewandte Physik
Technik Bauwesen
Technik Medizintechnik
Technik Umwelttechnik / Biotechnologie
Schlagworte biochemical engineering • Furoindolizine Core Skeleton • Indolizine-Based Seoul-Fluor System • Indolizine Core Skeleton • Molecular Fluorescence • Molecular Lipophilicity • Organic Fluorophores • Photophysical Parameters • SF-Based Lipid Droplet Bioprobes • Tetrazine-Containing Colorful Bioorthogonal Probes
ISBN-10 981-13-1645-7 / 9811316457
ISBN-13 978-981-13-1645-6 / 9789811316456
Haben Sie eine Frage zum Produkt?
PDFPDF (Wasserzeichen)
Größe: 11,6 MB

DRM: Digitales Wasserzeichen
Dieses eBook enthält ein digitales Wasser­zeichen und ist damit für Sie persona­lisiert. Bei einer missbräuch­lichen Weiter­gabe des eBooks an Dritte ist eine Rück­ver­folgung an die Quelle möglich.

Dateiformat: PDF (Portable Document Format)
Mit einem festen Seiten­layout eignet sich die PDF besonders für Fach­bücher mit Spalten, Tabellen und Abbild­ungen. Eine PDF kann auf fast allen Geräten ange­zeigt werden, ist aber für kleine Displays (Smart­phone, eReader) nur einge­schränkt geeignet.

Systemvoraussetzungen:
PC/Mac: Mit einem PC oder Mac können Sie dieses eBook lesen. Sie benötigen dafür einen PDF-Viewer - z.B. den Adobe Reader oder Adobe Digital Editions.
eReader: Dieses eBook kann mit (fast) allen eBook-Readern gelesen werden. Mit dem amazon-Kindle ist es aber nicht kompatibel.
Smartphone/Tablet: Egal ob Apple oder Android, dieses eBook können Sie lesen. Sie benötigen dafür einen PDF-Viewer - z.B. die kostenlose Adobe Digital Editions-App.

Buying eBooks from abroad
For tax law reasons we can sell eBooks just within Germany and Switzerland. Regrettably we cannot fulfill eBook-orders from other countries.

Mehr entdecken
aus dem Bereich