T-Cell Trafficking

Methods and Protocols

George Edward Rainger, Helen M. Mcgettrick (Herausgeber)

Buch | Softcover

252 Seiten

2018 | Softcover reprint of the original 2nd ed. 2017
Humana Press Inc. (Verlag)
978-1-4939-8342-1 (ISBN)

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This second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and maturation, traffic through secondary lymphoid organs during ‘physiological’ resolving inflammation and during immune responses, as well as T-Cell trafficking in chronic inflammatory diseases. Importantly, chapters cover methods from in silico modeling of cellular interactions, in vitro adhesion assays, through ex vivo functional assays to integrated intravital modeling of T-Cell trafficking through organs. Written in the highly successful Methods in Molecular Biology series format, each methods chapter includes a short introduction to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, T-Cell Trafficking: Methods and Protocols, Second Edition aims to be an essential point of reference for those new to the field of T-Cell trafficking, or to those looking to expand their technical capabilities.

Introduction to Homeostatic Migration.- Analysis of Thymocyte Migration, Cellular Interactions, and Activation by Multi-Photon Fluorescence Microscopy of Live Thymic Slices.- Visualising and Tracking T Cell Motility in vivo.- Graph Theory-based Analysis of the Lymph Node Fibroblastic Reticular Cell Network.- Introduction: T cell Trafficking in Inflammation and Immunity.- Leukocyte Adhesion under Haemodynamic Flow Conditions.- Endocrine Regulation of Lymphocyte Trafficking In Vitro.- Mesenchymal Stromal Cells as Active Regulators of Lymphocyte Recruitment to Blood Vascular Endothelial Cells.- Monitoring RhoGTPase Activity in Leukocytes using Classic “pull-down” Assays.- Utilising Lentiviral Gene Transfer in Primary Endothelial Cells to Assess Lymphocyte-Endothelial Interactions.- Introduction to Lymphocyte Trafficking in Disease.- Using Ex-Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking.- In vitro and Ex Vivo Models to Study T Cell Migration through the Human Liver Parenchyma.- Monitoring Migration of Activated T Cells to Antigen-rich Non-lymphoid Tissue.- Tissue Digestion for Stromal Cell and Leukocyte Isolation.- T-cell Response in the Lung Following Influenza Virus Infection.

Erscheinungsdatum	18.08.2018
Reihe/Serie	Methods in Molecular Biology ; 1591
Zusatzinfo	32 Illustrations, color; 15 Illustrations, black and white; XI, 252 p. 47 illus., 32 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
Schlagworte	Antigens • immune response • immune system • Lymphocytes • T-cell
ISBN-10	1-4939-8342-3 / 1493983423
ISBN-13	978-1-4939-8342-1 / 9781493983421
Zustand	Neuware