Single Nucleotide Polymorphisms
Humana Press Inc. (Verlag)
978-0-89603-968-1 (ISBN)
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Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
SNPs: Why Do We Care?Lisa D. BrooksDenaturing High-Performance Liquid ChromatographyAndreas Premstaller and Peter J. OefnerSNP Detection and Allele Frequency Determination by SSCPTomoko Tahira, Akari Suzuki, Yoji Kukita, and Kenshi HayashiConformation-Sensitive Gel ElectrophoresisArupa GangulyDetection of Mutations in DNA by Solid-Phase Chemical Cleavage Method: A Simplified AssayChinh T. Bui, Jeffrey J. Babon, Andreana Lambrinakos, and Richard G. H. CottonSNP Discovery by Direct DNA SequencingPui-Yan Kwok and Shenghui DuanComputational SNP Discovery in DNA Sequence DataGabor T. MarthGenotyping SNPs With Molecular BeaconsSalvatore A. E. Marras, Fred Russell Kramer, and Sanjay TyagiSNP Genotyping by the 5'-Nuclease ReactionKenneth J. LivakGenotyping SNPs by Minisequencing Primer Extension Using Oligonucleotide MicroarraysKatarina Lindroos, Ulrika Liljedahl, and Ann-Christine SyvanenQuantitative Analysis of SNPs in Pooled DNA Samples by Solid-Phase MinisequencingCharlotta Olsson, Ulrika Liljedahl, and Ann-Christine SyvanenHomogeneous Primer Extension Assay With Fluorescence Polarization DetectionTony M. Hsu and Pui-Yan KwokPyrosequencing for SNP GenotypingMostafa RonaghiHomogeneous Allele-Specific PCR in SNP GenotypingSoren Germer and Russell HiguchiOligonucleotide Ligation AssayJonas Jarvius, Mats Nilsson, and Ulf LandegrenInvader Assay for SNP GenotypingVictor Lyamichev and Bruce NeriMALDI-TOF Mass Spectrometry-Based SNP GenotypingNiels Storm, Brigitte Darnhofer-Patel, Dirk van den Boom, and Charles P. RodiIndex
| Erscheint lt. Verlag | 7.10.2002 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; v. 212 |
| Zusatzinfo | 60 black & white illustrations, 11 colour illustrations, 12 black & white halftones, 48 black & white line drawings |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 152 x 229 mm |
| Gewicht | 1 g |
| Einbandart | gebunden |
| Themenwelt | Medizin / Pharmazie ► Studium |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| ISBN-10 | 0-89603-968-4 / 0896039684 |
| ISBN-13 | 978-0-89603-968-1 / 9780896039681 |
| Zustand | Neuware |
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