Patch-Clamp Analysis

Advanced Techniques

Wolfgang Walz, Alan A. Boulton, Glen B. Baker (Herausgeber)

Buch | Hardcover

330 Seiten

2002
Humana Press Inc. (Verlag)
978-1-58829-013-7 (ISBN)

Infos

Describes applications that involve patch pipet. Beginning with basic patch-clamp techniques, this book presents the developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique.

---

"Patch-Clamp Analysis: Advanced Techniques" describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.

Inhalt

Technology of Patch-Clamp Electrodes. Whole-Cell Patch-Clamp Recordings. Single-Channel Recording. Combined Fluorometric and Electrophysiological Recordings. Patch-Clamp Techniques Applied to Brain Slices. Perforated Patch-Clamp Technique. Fast-Drug Application. Pipet Perfusion Methods: Techniques and Applications. Patch-Cram Detection of Cyclic GMP in Intact Cells. Loose-Patch Voltage-Clamp Technique. Recording from Macropatches. Combining Patch-Clamp Techniques with RT-PCR. Index.

Produkt-Details

• Erscheint lt. Verlag: 1.7.2002
• Reihe/Serie: Neuromethods ; v. 38
• Zusatzinfo: Illustrations
• Verlagsort: Totowa, NJ
• Sprache: englisch
• Maße: 152 x 229 mm
• Gewicht: 886 g
• Einbandart: gebunden
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Neurologie
• Themenwelt: Medizin / Pharmazie ► Studium
• ISBN-10: 1-58829-013-1 / 1588290131
• ISBN-13: 978-1-58829-013-7 / 9781588290137
• Zustand: Neuware