Meiosis

Buch | Softcover

337 Seiten

2018 | Softcover reprint of the original 2nd ed. 2017
Humana Press Inc. (Verlag)
978-1-4939-8169-4 (ISBN)

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This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailedand practical, Meiosis, Second Edition will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation.

Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae.- Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein-Binding in S. pombe.- Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast.- Ribosome Profiling for the Analysis of Translation During Yeast Meiosis.- Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation.- Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora.- High Throughput Screening to Identify Regulators of Meiosis-Specific Gene Expression in Saccharomyces cerevisiae.- Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast.- In Vivo Imaging of Budding Yeast Meiosis.- Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules During Meiotic Prophase I.- Flow Cytometry for theIsolation and Characterization of Rodent Meiocytes.- Imaging of Chromosome Dynamics in Mouse Testis Tissue by Immuno-FISH.- Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy.- Live Imaging of Meiosis I in Late Stage Drosophila melanogaster Oocytes.- Microscopy Methods for Analysis of Spindle Dynamics in Meiotic Drosophila Spermatocytes.- Drosophila Male Meiosis.- Analysis of Chromatin Dynamics During Drosophila Spermatogenesis.- Modeling of Meiotic Crossover Interference as Inspired by the Beam-Film Model.- A Computational Approach to Study Gene Expression Networks.

Erscheinungsdatum	16.08.2018
Reihe/Serie	Methods in Molecular Biology ; 1471
Zusatzinfo	45 Illustrations, color; 14 Illustrations, black and white; X, 337 p. 59 illus., 45 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
Schlagworte	Budding yeast models • Chromosome reorganization • computational methods • gametogenesis • gene expression networks • Meiotic chromosome crossovers
ISBN-10	1-4939-8169-2 / 1493981692
ISBN-13	978-1-4939-8169-4 / 9781493981694
Zustand	Neuware