Stem Cell-Derived Models in Toxicology

Mike Clements, Liz Roquemore (Herausgeber)

Buch | Softcover

341 Seiten

2018 | Softcover reprint of the original 1st ed. 2017
Humana Press Inc. (Verlag)
978-1-4939-8264-6 (ISBN)

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This detailed volume brings together a diverse collection of stem cell-derived model-based toxicity assays, from those routinely used to those deemed to have considerable potential. With a focus on differentiated tissues, the chapters explore numerous cardiotoxicity applications as well as coverage of neurotoxicity, hepatotoxicity, and more. Written for the Methods in Pharmacology and Toxicology series, the contents of this book aim to enable adoption of these protocols in laboratories that are interested in entering the field as well as to facilitate the transfer of best practices between laboratories that are already actively pursuing these technologies.
Authoritative and cutting-edge, Stem Cell-Derived Models in Toxicology serves as a vital resource for researchers aiming to improve risk assessment in drug discovery and design.

Stem Cell‐Derived Models for Safety and Toxicity Assessments: Present and Future Studies in the “Proclinical Space”.- Human Pluripotent Stem Cell Test for Assessing the Potential Teratogen Risk.- Cardiac Action Potential Measurement in Human Embryonic Stem Cell Cardiomyocytes for Cardiac Safety Studies Using Manual Patch-Clamp Electrophysiology.- Automated Patch Clamp Recordings of Human Stem Cell-Derived Cardiomyocytes.- The CiPA Microelectrode Array Assay with hSC-Derived Cardiomyocytes: Current Protocol, Future Potential.- Optogenetic Approach to Cardiotoxicity Screening: Simultaneous Voltage and Calcium Imaging Under Paced Conditions.- HTS Compatible Voltage- and Ca2+-Sensitive Dye Recordings from hiPSC-Derived Cardiomyocytes Using the Hamamatsu FDSS Systems.- Kinetic Image Cytometry for Predicting Arrhythmias Using Human Stem Cell-Derived Cardiomyocytes.- Decoding Ca2+ Signals as a Non-Electrophysiological Method forAssessing Drug Toxicity in Stem-Cell Derived Cardiomyocytes.- Combined Impedance and Extracellular Field Potential Recordings from Human Stem Cell-Derived Cardiomyocytes.- Edge Detection for Contractility Measurements with Cardiac Spheroids.- Contractile Force Readout of hESC-Cardiomyocytes.- In Vitro Cardiotoxicity Investigation Using High Content Analysis and Human Stem Cell-Derived Models.- Structural Toxicity: Hypertrophy Models of Human Pluripotent Stem Cell-Derived Cardiomyocytes.- Addressing Functional Neurotoxicity Using the Microelectrode Array (MEA).- Micropatterned Co-Cultures of Induced Pluripotent Stem Cell-Derived Hepatocytes and Stromal Cells for Drug Toxicity Studies.

Erscheinungsdatum	16.08.2018
Reihe/Serie	Methods in Pharmacology and Toxicology
Zusatzinfo	108 Illustrations, color; 16 Illustrations, black and white; XII, 341 p. 124 illus., 108 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie
Themenwelt	Medizin / Pharmazie ► Pharmazie
Schlagworte	cardiomyocytes • Cardiotoxicity • drug development • Hepatotoxicity • neurotoxicity • Pluripotent stem cell models • Pre-clinical safety assessments
ISBN-10	1-4939-8264-8 / 1493982648
ISBN-13	978-1-4939-8264-6 / 9781493982646
Zustand	Neuware