Site-Specific Recombinases

Methods and Protocols

Buch | Softcover

342 Seiten

2018 | Softcover reprint of the original 1st ed. 2017
Humana Press Inc. (Verlag)
978-1-4939-8405-3 (ISBN)

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This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.

Generating Genetically Modified Mice: A Decision Guide.- Direct Generation of Conditional Alleles using CRISPR/Cas9 in Mouse Zygotes.- Building Cre Knockin Rat Lines using CRISPR/Cas9.- Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases.- Use of the DICE Dual Integrase Cassette Exchange System.- Ligand-controlled Site-specific Recombination in Zebrafish.- Injection-based Delivery of Cell Permeable Peptide-tagged Cre.- Viral Delivery of GFP-dependent Recombinases to the Mouse Brain.- Recombinase-mediated Cassette Exchange using Adenoviral Vectors.- Marker Removal in Transgenic Plants using Cre recombinase Delivered with Potato Virus X.- Nanoparticle-mediated Recombinase Delivery into Maize.- Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines.- FLPing Genes On and Off in Drosophila.- Imaging Neural Architecture in Brainbow Samples.- Purification and in vitro characterization.

Erscheinungsdatum	09.07.2018
Reihe/Serie	Methods in Molecular Biology ; 1642
Zusatzinfo	25 Illustrations, color; 25 Illustrations, black and white; XI, 342 p. 50 illus., 25 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 1. Studienabschnitt (Vorklinik) ► Anatomie / Neuroanatomie
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
ISBN-10	1-4939-8405-5 / 1493984055
ISBN-13	978-1-4939-8405-3 / 9781493984053
Zustand	Neuware