Tertiary Lymphoid Structures

Methods and Protocols

Marie-Caroline Dieu-Nosjean (Herausgeber)

Buch | Hardcover

289 Seiten

2018 | 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-8708-5 (ISBN)

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This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations. Pre-clinical models are also discussed in detail to show how TLS structure, development, and maintenance can be targeted and studied in vivo. The chapters in this book cover topics such as humans and mice; strategies to quantify TLS in order to use it in stained tissue sections; classifying a gene signature form fixed and paraffin-embedded tissues; and development of murine inflammatory models to help look at TLS in the context of infection or malignancy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Tertiary Lymphoid Structures: Methods and Protocols is a valuable resource that increases the reader’s knowledge on immune functions and how they will pave the way to future therapeutic applications.

Tertiary Lymphoid Structures among the World of Non-Canonical Ectopic Lymphoid Organizations.- Cellular and Vascular Components of Tertiary Lymphoid Structures.- Meningeal Immunity, Drainage, and Tertiary Lymphoid Structure Formation.- Development of Tools for the Selective Visualization and Quantification of TLS-Immune Cells on Tissue Sections.- A Quantitative Pathology Approach to Analyze the Development of Human Cancer-Associated Tertiary Lymphoid Structures.- Multiplex Immunohistochemistry for Image Analysis of Tertiary Lymphoid Structures in Cancer.- Defining High Endothelial Venules and Tertiary Lymphoid Structures in Cancer.- Development of Methods for Selective Gene Expression Profiling in Tertiary Lymphoid Structure using Laser Capture Microdissection.- Quantifying Tertiary Lymphoid Structure-Associated Genes in Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues.- Generation of Recombinant Monoclonal Antibodies from Single B Cells Isolated from Synovial Tissue of Rheumatoid Arthritis Patients.- Designed Methods for the Sorting of Tertiary Lymphoid Structure-Immune Cell Populations.- Identification of Tertiary Lymphoid Structure-Associated Follicular Helper T Cells in Human Tumors and Tissues.- Exploring the Role of Tertiary Lymphoid Structures using a Mouse Model of Bacteria-Infected Lungs.- Identification and Characterization of Tertiary Lymphoid Structures in Murine Melanoma.- Investigating Tumor-Associated Tertiary Lymphoid Structures in Murine Lung Adenocarcinoma.- Targeting Tertiary Lymphoid structures for Tumor Immunotherapy.

Erscheinungsdatum	18.09.2018
Reihe/Serie	Methods in Molecular Biology ; 1845
Zusatzinfo	34 Illustrations, color; 4 Illustrations, black and white; XII, 289 p. 38 illus., 34 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
Schlagworte	Cancer Research • CD8+ T cells • endothelial venules • Immune Function • immunotherapy • neogenesis
ISBN-10	1-4939-8708-9 / 1493987089
ISBN-13	978-1-4939-8708-5 / 9781493987085
Zustand	Neuware