Rat Hybridomas and Rat Monoclonal Antibodies (1990)

Herve Bazin (Autor)

Buch | Hardcover

534 Seiten

2017
CRC Press (Verlag)
978-1-138-50610-7 (ISBN)

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The purpose of this book is to provide information which supports the fact that rat hybridomas are no more difficult to develop than mouse hybridomas. This is the first book devoted to the development of rat hybridomas. It includes theories, step-by-step techniques, ingredients and apparatus. The focus of this work is on the antibody repertoire, the unique biological properties of rat immunoglobulins, the one-step purification procedure by immunoaffinity chromatography, the absence of C-type particles, and the easy production of large amounts of ascitic fluid containing rat MAb. This rare publication is an absolute must for all scientists using MAbs and those interested in the fields of immunology, biotechnology, and biochemistry.

Herve Bazin

Introduction

Part I IMMUNOCYTOMAS in LOUVAIN (LOU) RATS.

Chapter 1

Rat Immuoglobulins.

Chapter 2

The LOUVAIN (LOU) Rats.

Chapter 3

Rat Immunocytomas (IR).

Part II PRODUCTION of RAT-RAT HYBRIDOMAS.

Chapter 4

Fusion Cell Lines.

Chapter 5

Immunization of Rats.

Chapter 6.I

Fusion Procedure.

Chapter 6.II

Rat Hybridoma Prodcution: Scanning electron Microscopy of a Fusion Experiment

Chapter 7

Selective Growth of Hybrids.

Chapter 8

Cloning.

Chapter 9.I

Screening of Monoclonal Antibodies Directed Against Soluble Antigens

Chapter 9.II

Screening of Hybrid Supernatants for Monoclonal Antibodies Against Cellular Antigens

Chapter 10

Isotyping of Rat Monoclonal Antibodies.

Chapter 11

Adaptation to In Vivo Culture. Freezing and Thawing.

Chapter 12

Freezing and Thawing

Part III PRODUCTION, PURIFICATION, FRAGMENTATION and LABELING of MONOCLONAL ANTIBODIES.

Chapter 13

In Vitro Production of Rat Monoclonal Antibodies.

Chapter 14

In Vivo Production of Rat Monoclonal Antibodies.

Chapter 15

Purification of Rat Monoclonal Antibodies from Ascitic Fluid - Serum or Culture Supernatant.

Chapter 16

Quality Control of Purified Antibodies.

Chapter 17

Fragmentation of Rat Monoclonal Antibodies.

Chapter 18

Labeling of Monoclonal Antibodies.

Part IV EXAMPLES of PRODUCTION and CHARACTERIZATION of RAT-RAT HYBRIDOMAS AGAINST VARIOUS Ag.

Chapter 19.I

Rat Monoclonal Antibodies Against Murine Immunoglobulins.

Chapter 19.II

Allotype Specific Monoclonal Antibodies to Rat Kappa Light Chain.

Chapter 19.III

Application of Rat Hybridomas to Studies on Ocular Immunobiology.

Chapter 19.IV

Rat Monoclonal Antibodies Against Rabbit Immunoglobulin.

Chapter 19.V

Preparation of Anti-Human IgG Monoclonal Antibodies Using Rat x Rat Hybridoma System.

Chapter 19.VI

Rat Monoclonal Antibodies Against Human Immunoglobulin.

Chapter 19.VII

Production and Characterization of Rat Monoclonal Antibodies Directed Against Human Apolipoprotein B.

Chapter 19.VIII

Studies on Rat-Rat Hybridoma Technique and its Application to Obtain Rat Monoclonal Antibodies Anti-Horse-radish Peroxidase.

Chapter 19.IX

Rat-Rat and Rat-Mouse Hybridomas Secreting Rat Monoclonal Anti-Horse-radish Peroxidase.

Chapter 19.X

LOU Rat Monoclonal Antibodies Against Xenopus Vitellogenin.

Chapter 19.XI

Production and Characterization of Rat-Rat Hybridomas Against DNP-Hapten. Rat x Rat Hybridomas Against Cellular Antigens.

Chapter 20.I

Rat Monoclonal Antibodies Against Human Leukocyte Differentiation Antigens.

Chapter 20.II

Rat Monoclonal Antibodies Specific for T Lymphocytes.

Chapter 21.I

Rat Monoclonal Antibodies Against Streptococcus mutans and Bacteroides gingivalis.

Chapter 21.II

A Specific Rat Monoclonal Antibody Against Yersinia enterocolitica Serogroup 0:8.

Chapter 21.III

Production of Rat Hybridoma Directed Against Yersinia enterocolitica.

Chapter 22

Rat Monoclonal Antibodies Against Viral Antigens.

Chapter 23

Contribution of Rat Monoclonal Antibodies to the Study of Schistosoma mansoni Infections.

Part V SPECIFIC USES and PROPERTIES of RAT MONOCLONAL ANTIBODIES.

Chapter 24.I

Mouse Immunoglobulin Isotyping.

Chapter 24.II

Humoral Immune Suppression of Mice by Monoclonal Rat Anti-Mouse µHeavy Chain Antibodies.

Chapter 24.III

Rat Monoclonal Antibodies Against Mouse IgE.

Chapter 24.IV

Purification of Mouse Monoclonal Antibodies by Immunoaffinity Chromatography.

Chapter 24.V

Screening of Mouse Monoclonal Antibodies.

Chapter 24.VI

Use of Rat Monoclonal Antibodies in Immunohistochemistry Investigations.

Chapter 24.VII

Use of Rat-Rat Monoclonal Antibodies Labeled with Fluorochrome.

Chapter 25.I

Use of Rat Monoclonal Anti-Rat Allotype Antibodies in Immunohistoperoxidase Technique and ELISA.

Chapter 25.II

Use of Anti-Rat Kappa Light Chain Allotype Monoclonal Antibodies for the Quantification of Rat Monoclonal Antibodies in Ascitic Fluid or Serum.

Chapter 26

Extracorporeal Removal of Arteriogenic Particles by Use of Rat Monoclonal Antibody Coupled to Sepharose as Specific Immunosorbent.

Chapter 27

Antibacteria Antigens, Especially Streptococcus mutans.

Chapter 28

Mercury-Induced Autoimmunity Production of Monoclonal Autoantibodies.

Chapter 29

Rat Monoclonal Antibodies to Plant Pathogens.

Chapter 30.I

Rat Monoclonal Antibodies for Complement-Mediated and Cell-Medicated Cytolysis.

Chapter 30.II

Rat Antibodies and K Cells.

Chapter 31

Absence of Viral Particles in Rat Hybridomas - An Ultrastructural Study.

Chapter 32

Role of CODATA-HDB in the Centralization and Distribution of Information Concerning the Rat Hybridomas.

Chapter33

List of Monoclonal Antibodies.

Erscheinungsdatum	19.12.2017
Reihe/Serie	CRC Press Revivals
Zusatzinfo	87 Tables, black and white; 34 Halftones, black and white
Verlagsort	London
Sprache	englisch
Maße	178 x 254 mm
Gewicht	453 g
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zoologie
ISBN-10	1-138-50610-9 / 1138506109
ISBN-13	978-1-138-50610-7 / 9781138506107
Zustand	Neuware