Host-Fungus Interactions
Humana Press Inc. (Verlag)
978-1-4939-5904-4 (ISBN)
Comprehensive and practical, Host-Fungus Interactions: Methods and Protocols describes available molecular methods and fungal infection models in great detail in order to encourage researchers to try new approaches to investigating host-fungus interactions with added levels of confidence.
Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy.- Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans.- Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans.- Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi.- Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers.- Multiple Gene Deletion in Cryptococcus neoformans Using the Cre--lox System.- Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast.- Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach.- Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers.- RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum.- RNA Interference in Cryptococcus neoformans.- Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA.- Tetracycline-Inducible Gene Expression in Candida albicans.- Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii.- Modular Gene Over-Expression Strategies for Candida albicans.- Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans.- Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans.- Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils.- Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions.- Simple Assays for Measuring Innate Interactions with Fungi.- Binding and Uptake of Candida albicans by Human Monocyte-Derived Dendritic Cells.-Immune Responses to Candida albicans in Models of In Vitro Reconstituted Human Oral Epithelium.- Analysis of Host-Cell Responses by Immunoblotting, ELISA, and Real-Time PCR.- In Vitro Model of Invasive Pulmonary Aspergillosis in the Human Alveolus.- Biofilm Formation Studies in Microtiter Plate Format.- Transcript Profiling Using ESTs from Paracoccidioides brasiliensis in Models of
Infection.- Laser Capture Microdissection of Candida albicans from Host Tissue.- Isolation and Amplification of Fungal RNA for Microarray Analysis from Host Samples.- Cytokine Measurement Using Cytometric Bead Arrays (CBA).- Transcript Profiling of the Murine Immune Response to Invasive Aspergillosis.- Caenorhabditis elegans: A Nematode Infection Model for Pathogenic Fungi.- Drosophila melanogaster as a Model Organism for Invasive Aspergillosis.- Galleria mellonella as a Model for Fungal Pathogenicity Testing.- Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi.- Mouse Intravenous Challenge Models and Applications.- A Nebulized Intra-Tracheal Rat Model of Invasive Pulmonary Aspergillosis.- Invasive Models of Histoplasmosis.- Murine Model of Concurrent Oral and Vaginal Candida albicans Colonization.- A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections.- Modeling of Fungal Biofilms Using a Rat Central-Vein Catheter.- Orogastrointestinal Model of Mucosal and Disseminated Candidiasis.- A Non-Lethal Murine Cutaneous Model of Invasive Aspergillosis.
Erscheinungsdatum | 19.08.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 845 |
Zusatzinfo | 37 Illustrations, color; 67 Illustrations, black and white; XVI, 597 p. 104 illus., 37 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin |
Studium ► 2. Studienabschnitt (Klinik) ► Pathologie | |
Naturwissenschaften ► Biologie ► Allgemeines / Lexika | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-4939-5904-2 / 1493959042 |
ISBN-13 | 978-1-4939-5904-4 / 9781493959044 |
Zustand | Neuware |
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