DNA Topoisomerases

Methods and Protocols

Buch | Hardcover

328 Seiten

2017 | 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-7458-0 (ISBN)

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This volume looks at the evolution of the topo field over the last year and discusses how DNA topoisomerases are directly and indirectly involved in numerous cellular processes. The chapters in this book cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental protocols related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; protocols for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmid-mediated quinolone resistance in bacteria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, DNA Topoisomerases: Methods and Protoc

ols is intended for specialists and non-specialists with an overview of selected hot topics in the field, and the latest protocols to study DNA topology, topoisomerases functions and inhibition by various compounds.

Type IA DNA Topoisomerases: A Universal Core and Multiple Activities.- Topoisomerase I and Genome Stability: The Good and The Bad.- DNA Topoisomerases as Targets for Antibacterial Agents.- DNA Supercoiling Measurement in Bacteria.- DNA Catenation Reveals the Dynamics of DNA Topology during Replication.- Mapping E. coli Topoisomerase IV Binding and Activity Sites.- The Use of Psoralen Photobinding to Study Transcription-Induced Supercoiling.- Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast.- Detection of oriC-Independent Replication in Escherichia coli Cells.- Single-Molecule Magnetic Tweezer Analysis of Topoisomerases.- Synthesis of Hemicatenanes for the Study of Type IA Topoisomerases.- An Assay for Detecting RNA Topoisomerase Activity.- Studying TDP1 Function in DNA Repair.- Topoisomerase II Chromatin Immunoprecipitation.- Analyzing Mitotic Chromosome Structural Defects after Topoisomerase II Inhibition or Mutation.- Monitoring the DNA Topoisomerase II Checkpoint inSaccharomyces cerevisiae.- Studying Topoisomerase 1-Mediated Damage at Genomic Ribonucleotides.- A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons.- Fluoroquinolone-Gyrase-DNA Cleaved Complexes.- Detection of Topoisomerase Covalent Complexes in Eukaryotic Cells.- Visualization and Quantification of Topoisomerase-DNA Covalent Complexes Using the Trapped in Agarose Immunostaining (TARDIS) Assay.- Study of Plasmid-Mediated Quinolone Resistance in Bacteria.

Erscheinungsdatum	30.01.2018
Reihe/Serie	Methods in Molecular Biology ; 1703
Zusatzinfo	21 Illustrations, color; 46 Illustrations, black and white; XI, 328 p. 67 illus., 21 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
Schlagworte	chromosome segregation • Cre/LoxP • Genome Stability • Purification • supercoiled DNA • topoisomerases
ISBN-10	1-4939-7458-0 / 1493974580
ISBN-13	978-1-4939-7458-0 / 9781493974580
Zustand	Neuware