Parasite Genomics Protocols

Buch | Softcover

367 Seiten

2017 | Softcover reprint of the original 2nd ed. 2015
Humana Press Inc. (Verlag)
978-1-4939-4220-6 (ISBN)

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Parasite Genomics Protocols, Second Edition expands upon the previous edition with current, detailed methodologies that have been adapted to research parasites in the post genomic era. With new chapters on parasite studies, helminth pathogens, and protocols for undertaking other large-scale ‘omics’ methodologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Parasite Genomics Protocols, Second Edition offers a wide-ranging collection of diverse methods and protocols for further study into these unique organisms.

The Eukaryotic Pathogen Databases: A Functional Genomic Resource Integrating Data from of Human and Veterinary Parasites.- From Sequence Mapping to Genome Assemblies.- Sequencing and Annotation of Mitochondrial Genomes from Individual Parasitic Helminths.- A Beginners Guide to Estimating the Non-synonymous to Synonymous Rate Ratio of all Protein-coding Genes in a Genome.- Exploiting Genetic Variation to Discover Genes Involved in Important Disease Phenotypes.- Identification and Analysis of Ingi-Related Retroposons in the Trypanosomatid Genomes.- Approaches for Studying mRNA Decay Mediated by SIDER2 Retroposons in Leishmania.- Gene Suppression in Schistosomes using RNAi.- Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Transcript Ends.- Techniques to Study Epigenetic Control and the Epigenome in Parasites.- The Genome-wide Identification of Promoter Regions in Toxoplasma gondii.- RNA-seq Approaches for Determining mRNA Abundance in Leishmania.- Protein Microarrays for Parasite Antigen Discovery.- A Transposon-based Tool for Transformation and Mutagenesis in Trypanosomatid Protozoa.- Separation of Basic Proteins from Leishmania using a combination of Free Flow Electrophoresis (FFE) and 2D Electrophoresis (2-DE) under Basic Conditions.- Proteomic Analysis of Post Translational Modifications using iTRAQ in Leishmania.- Large Scale Differential Proteome Analysis in Plasmodium falciparum under Drug Treatment.- Use of 13C Stable Isotope Labelling for Pathway and Metabolic Flux Analysis in Leishmania Parasites.- Molecular Genotyping of Trypanosoma cruzi for Lineage Assignment and Population Genetics.- Screening Leishmania donovani Complex-specific Genes Required for Visceral Disease.

Erscheinungsdatum	23.03.2017
Reihe/Serie	Methods in Molecular Biology ; 1201
Zusatzinfo	34 Illustrations, color; 15 Illustrations, black and white; XII, 367 p. 49 illus., 34 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	epigenome • genetic variation • genome data • helminth pathogens • human disease • large-scale ‘omics’ methodologies • Metabolome • Parasites • post genomic era • Proteome • protozoan • Sequence data • static data repositories • transcriptome
ISBN-10	1-4939-4220-4 / 1493942204
ISBN-13	978-1-4939-4220-6 / 9781493942206
Zustand	Neuware