Zygotic Genome Activation
Humana Press Inc. (Verlag)
978-1-4939-6986-9 (ISBN)
Thorough and cutting-edge, Zygotic Genome Activation: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA.
Clearance of Maternal RNAs: Not a Mummy’s Embryo Anymore.- Link of Zygotic Genome Activation and Cell Cycle Control.- Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA during Embryogenesis.- Gene Expression Analysis of Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR.- Detection of miRNA in Mammalian Oocytes and Embryos.- Detection of Bidirectional Promoter-Derived IncRNAs from Small-Scale Samples using Pre-Amplification-Free Directional RNA-Seq Method.- Detection and Characterization of Small Non-Coding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation.- Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos.- RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos.- Detection of RNA Polymerase II in Mice Embryos during Zygotic Genome Activation using Immunocytochemistry.- Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo.- Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos.- Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation.- Targeted Gene Knockdown in Early Embryos using siRNA.- Generating Mouse Models using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput.- CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine.- Potential Involvement of SCF-Complex in Zygotic Genome Activation during Early Bovine Embryo Development.- Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes.
| Erscheinungsdatum | 17.05.2017 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1605 |
| Zusatzinfo | 41 Illustrations, color; 10 Illustrations, black and white; XI, 272 p. 51 illus., 41 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
| Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
| Schlagworte | CRISPR • Embryogenesis • epigenetics • Genetic Engineering • ZGA • Zygote |
| ISBN-10 | 1-4939-6986-2 / 1493969862 |
| ISBN-13 | 978-1-4939-6986-9 / 9781493969869 |
| Zustand | Neuware |
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