Methods in Cellular Immunology, Second Edition

Rafael Fernandez-Botran, Vaclav Vetvicka (Autoren)

Buch | Softcover

246 Seiten

1995
Crc Press Inc (Verlag)
978-0-8493-6548-5 (ISBN)

Infos

This guide to the most common cellular immunological methods looks at both human and murine models, and presents descriptions of the most common techniques. Background information on the techniques is given to provide a wider understanding of the evolution of the various methods.

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Methods in Cellular Immunology provides a guide to cellular immunological methods. This indispensible book covers both murine and human models. The described methods involve cell isolation and separation; antibody production, detection, and isolation; flow cytometry; NK cytotoxicity; and quantitation of cytokines and cytokine receptors.This practical, easy-to-follow methods book presents step-by-step descriptions of the most common techniques of cellular immunology in a style that both novices and seasoned scientists will be able to understand. Text includes the background information necessary to perform basic techniques. An extensive list of references is provided, allowing readers to compare the methods in the book with the original techniques. The book also lists the manufacturers of all chemicals and materials used in the procedures.
Both scientists and researchers involved in basic and clinical research and all scientists wishing to employ immunological techniques will benefit from this valuable resource.

Inhalt

Cells
Preparation of Cell Suspensions
Isolation of Cells
References
Flow Cytometry
Proliferation Assays
Incorporation of Tritiated Thymidine into DNA
Nonradioactive Detection Methods (MTT Assay)
References
Preparation of Cell Clones
Preparation of Mouse T Cell Clones
Preparation of Human T Cell Clones
Preparation of Human B Cell Clone
Cloning by Limiting Dilution Technique
References
Cytotoxic Cells
Cell-Mediated Cytotoxicity
References
Assays of Cytokines
Introduction
Bioassays
Immunoassays
References
Cytokine Receptors
Preparation and Modification of Antibodies
Polyclonal Antibodies
Monoclonal Antibodies
Coupling of Synthetic Peptides to Carrier Proteins
References
Detection of Antibodies
Hemolytic Plaque Assays
ELISA
References
Purification of Immunoglobulins
Precipitation by Ammonium Sulfate
Caprylic Acid-Ammonium Sulfate Isolation of IgG from Ascites Fluid
Purification of Antibodies by Precipitation with Polyethylene Glycol
Purification of Antibodies from Culture Supernatants by Precipitation with Polyethylene Glycol
Affinity Chromatography Using Protein A-Sepharose
Purification of IgM
Ammonium Sulfate Precipitation of IgM
Purification of Anti-Hapten Antibodies by Affinity Chromatography
Fragmentation of Immunoglobulin into F(ab) Fragments using Papain
Fragmentation of Immunoglobulin into F(ab) Fragments using Preactivated Papain
Fragmentation of Immunoglobulin into F(ab)2 Fragments using Pepsin
Double Immunodiffusion Assay
References
Buffers and Media
Commercial Sources
Index

Produkt-Details

• Erscheint lt. Verlag: 27.6.1995
• Zusatzinfo: 6 Tables, black and white
• Verlagsort: Bosa Roca
• Sprache: englisch
• Maße: 156 x 235 mm
• Gewicht: 372 g
• Einbandart: Paperback
• Themenwelt: Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• ISBN-10: 0-8493-6548-1 / 0849365481
• ISBN-13: 978-0-8493-6548-5 / 9780849365485
• Zustand: Neuware