Cardiac Tissue Engineering

Methods and Protocols

Milica Radisic, Lauren D. Black III (Herausgeber)

Buch | Softcover

266 Seiten

2016 | Softcover reprint of the original 1st ed. 2014
Humana Press Inc. (Verlag)
978-1-4939-4613-6 (ISBN)

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Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.

Second Generation Codon Optimized Minicircle (Comic) For Non-Viral Reprogramming Of Human Adult Fibroblasts.- Scalable Cardiac Differentiation Of Human Pluripotent Stem Cells as Microwell-Generated, Size Controlled Three-Dimensional Aggregates.- Preparation And Characterization Of Circulating Angiogenic Cells For Tissue Engineering Applications.- Isolation And Expansion Of C-Kit Positive Cardiac Progenitor Cells By Magnetic Cell Sorting.- Synthesis Of Aliphatic Polyester Hydrogel For Cardiac Tissue Engineering.- Fabrication Of Pegylated Fibrinogen, A Versatile Injectable Hydrogel Biomaterial.- Native Cardiac Extracellular Matrix Hydrogels For Cultivation Of Human Stem Cell-Derived Cardiomyocytes.- Magnetically-Actuated Alginate Scaffold: A Novel Platform For Promoting Tissue Organization And Vascularisation.- Shrink-Induced Biomimetic Wrinkled Substrates For Functional Cardiac Cell Alignment and Culture.- Injectable ECM Scaffolds For Cardiac Repair.- Generation Of Strip-Format Fibrin-Based Engineered Heart Tissue (EHT).- Cell Tri-Culture For Cardiac Vascularization.- Cell Sheet Technology For Cardiac Tissue Engineering.- Design And Fabrication Of Biological Wires.- Collagen-Based Engineered Heart Muscle.- Creation Of A Bioreactor For The Application Of Variable Amplitude Mechanical Stimulation Of Fibrin-Gel Based Engineered Cardiac Tissue.- Preparation Of Acellular Myocardial Scaffolds With Well-Preserved Cardiomyocyte Lacunae, And Method For Applying Mechanical And Electrical Simulation To Tissue Construct.- Patch-Clamp Technique In ESC-Derived Cardiomyocytes.- Optogenetic Control Of Cardiomyocytes.- Methods For Assessing The Electromechanical Integration Of Human Pluripotent Stem Cell-Derived Cardiomyocyte Graft.- Quantifying Electrical Interactions Between Cardiomyocytes and Other Cells In Micropatterned Cell Pairs.

Erscheinungsdatum	08.10.2016
Reihe/Serie	Methods in Molecular Biology ; 1181
Zusatzinfo	60 Illustrations, color; 17 Illustrations, black and white; XII, 266 p. 77 illus., 60 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Biomedizin
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	biomaterial patches • biomaterial preparation • cardiac patch • cardiac tissue engineering • Cell preparation • cell seeding • cultivation in various systems • engineering of myocardial wall in vitro • Human hearts • Hydrogels • Myocardial Infarction • myocardial patch • myocardial regeneration • myocardial wall in vitro
ISBN-10	1-4939-4613-7 / 1493946137
ISBN-13	978-1-4939-4613-6 / 9781493946136
Zustand	Neuware