Pseudogenes

Functions and Protocols

Buch | Softcover

319 Seiten

2016 | Softcover reprint of the original 1st ed. 2014
Humana Press Inc. (Verlag)
978-1-4939-4646-4 (ISBN)

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Providing a list of methods useful both to those who wish to study pseudogenes and to those who actually want to avoid their inadvertent detection, Pseudogenes: Functions and Protocols explores techniques involving pseudogenic DNA, RNA, and peptides/proteins, once believed to lack any functionality, but now known to be involved in complex regulatory circuits. After a few introductory chapters that overview the functions so far attributed to pseudogenes, this thorough volume delves into methods for pseudogene identification, for the detection of pseudogene transcription and translation, and for the study of the functions of pseudogenic RNA and proteins, as well as methods to avoid pseudogene detection when the focus of the research is their highly homologous parental counterparts. As part of the highly successful Methods in Molecular Biology series, chapters feature the kind of detailed descriptions and implementation advice that ensures successful results in the lab.

Authoritative and practical, Pseudogenes: Functions and Protocols will contribute to the high interest of the scientific community toward pseudogenes, while stimulating the conception of pseudogene - centered research projects and providing experimental protocols that can facilitate their execution.

Computational Methods for Pseudogene Annotation Based on Sequence Homology.- Computational Methods of Identification of Pseudogenes Based on Functionality: Entropy and GC Content.- Computational Methods of Identification of Pseudogenes Based on Functionality: Hybridization of 18S rRNA and mRNA During Translation.- Whole Genome Identification of Neutrally Evolving Pseudogenes Using the Evolutionary Measure dN/dS.- Methods to Study the Occurrence and the Evolution of Pseudogenes Through a Phylogenetic Approach.- Methods for Detecting Transcribed Pseudogenes: PCR on Regions of High Sequence Similarity Followed by Cloning and Sequencing,0 RNA Amplification for Pseudogene Detection Using RNA-Seq.- GENCODE Pseudogenes.- Methods to Detect Transcribed Pseudogenes: RNA-Seq Discovery Allows Learning Through Features.- Proteomics Techniques for the Detection of Translated Pseudogenes.- Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation.- Pseudogenes: A Novel Source of Trans Acting Antisense RNAs.- Pseudogene-Derived Endogenous siRNAs and Their Function.- Methods to Study Translated Pseudogenes: In Vitro Translation, Fusion with a Tag/Reporter Gene, and Complementation Assay.- Targeted and Robust Amplification of Mitochondrial DNA in the Presence of Nuclear Encoded Mitochondrial Pseudogenes Using Φ29 DNA Polymerases.- Discrimination of Pseudogene and Parental Gene DNA Methylation Using Allelic Bisulphite Sequencing Mutational Analysis of CYP21A2 Gene and CYP21A1P Pseudogene: Long Range PCR on Genomic DNA.- PMS2 Gene Mutational Analysis: RNA-Based Direct cDNA Sequencing to Circumvent Pseudogene Interference.- Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era.

Erscheinungsdatum	08.10.2016
Reihe/Serie	Methods in Molecular Biology ; 1167
Zusatzinfo	38 Illustrations, color; 20 Illustrations, black and white; X, 319 p. 58 illus., 38 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
Schlagworte	Inadvertent detection • Non-coding genome • Pseudogene identification • Pseudogenic DNA and RNA • Pseudogenic proteins • Regulatory long non-coding RNAs • Transcribed pseudogenes
ISBN-10	1-4939-4646-3 / 1493946463
ISBN-13	978-1-4939-4646-4 / 9781493946464
Zustand	Neuware