Legume Genomics

Methods and Protocols

Buch | Softcover

316 Seiten

2016 | Softcover reprint of the original 1st ed. 2013
Humana Press Inc. (Verlag)
978-1-4939-6024-8 (ISBN)

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Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.

The Model Legume Genomes.- Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization.- Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes.- RNA-Seq for Transcriptome Analysis in Non-Model Plants.- Functional Analysis of Legume Genome Arrays.- Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing.- Determining Abundance of MicroRNAs and Other Small RNAs in Legumes.- Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines.- Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection.- High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants.- Isolation and Forward Genetic Analysis of Developmental Genes in Pea.- Gene Silencing in Medicago truncatula Roots Using RNAi.- Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius).- Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens.- Transient Gene Expression in Medicago truncatula Leaves Via Agroinfiltration.- Subcellular Localization of Transiently-Expressed Fluorescent Fusion Proteins.- Proteomics and the Analysis of Nodulation.- Phosphoproteomic Analysis of Peptides.- Plant Metabolomics: From Experimental Design to Knowledge Extraction.

Erscheinungsdatum	04.10.2016
Reihe/Serie	Methods in Molecular Biology ; 1069
Zusatzinfo	23 Illustrations, color; 30 Illustrations, black and white; XI, 316 p. 53 illus., 23 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Botanik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
ISBN-10	1-4939-6024-5 / 1493960245
ISBN-13	978-1-4939-6024-8 / 9781493960248
Zustand	Neuware