Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
Humana Press Inc. (Verlag)
978-1-4939-6516-8 (ISBN)
Authoritative and cutting-edge, Eukaryotic Transcription and Post-Transcription Gene Expression Regulation aims to ensure successful results in the further study of this vital field.
Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators.- Tandem Affinity Purification Approach Coupled To Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-binding Proteins.- Efficient Preparation of High-Complexity ChIP-Seq Profiles from Early Xenopus Embryos.- Systematic Discovery Of Chromatin-bound Protein Complexes from ChIP-seq Datasets.- Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-seq) Protocol for Zebrafish Embryos.- Establishment of time- and cell-specific RNAi in Caenorhabditis elegans.- Cell-penetrating Peptide-mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing.- Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets.- eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response.- High Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA using 4-thiouridine.- Accurate Detection of Differential Expression and Splicing using Low-level Features.- Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry.- Determining if an mRNA is a Substrate of Nonsense-mediated mRNA Decay in Saccharomyces cerevisiae.- Optimizing In Vitro Pre-mRNA 3' Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HeLa Cleavage Factors and from Adherent HeLa Cell Nuclear Extract.- Unbiased Interrogation of 3D Genome Topology using Chromosome Conformation Capture Coupled to High-throughput Sequencing (3C-Seq).- Using an Inducible CRISPR-Cas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells.- In vitro assay to Study Histone Ubiquitination during Transcriptional Regulation.- Determination of Alternate Splicing Events using Transcriptome Arrays.- Large-scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor.- GeneTranscriptional Analysis-based Integrative Genomics Approach to Identify Tumor Promoting Metabolic Genes.
| Erscheinungsdatum | 30.11.2016 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1507 |
| Zusatzinfo | 29 Illustrations, color; 20 Illustrations, black and white; XI, 278 p. 49 illus., 29 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
| Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
| Schlagworte | Eukaryotic cells • eukaryotic gene expression regulation • gene expression • posttranscriptional gene expression regulation • transcriptional |
| ISBN-10 | 1-4939-6516-6 / 1493965166 |
| ISBN-13 | 978-1-4939-6516-8 / 9781493965168 |
| Zustand | Neuware |
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