Blick ins Buch

RNA Vaccines

Methods and Protocols

Thomas Kramps, Knut Elbers (Herausgeber)

Buch | Hardcover

282 Seiten

2016 | 1st ed. 2017
Humana Press Inc. (Verlag)
978-1-4939-6479-6 (ISBN)

Lese- und Medienproben

Blick ins Buch (midvox)

Artikel merken

This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, RNA Vaccines: Methods and Protocols aims to increased collaboration on RNA vaccines between basic and applied scientists in academia, government, and industry to develop future solutions for today’s challenges.

Introduction to RNA Vaccines.- Self-replicating RNA.- Self-replicating RNA Vaccine Delivery to Dendritic Cells.- Plant Expression of Trans-encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons.- RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines.- Nucleoside Modified mRNA Vaccines for Infectious Diseases.- Generation and Evaluation of Prophylactic mRNA Vaccines against Allergy.- Measuring the Adjuvant Activity of RNA Vaccines.- Generation of Immunostimulating 130 nm Protamine-RNA Nanoparticles.- Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins.- Adjuvant-Enhanced mRNA Vaccines.- Enhanced Delivery of DNA or RNA Vaccines by Electroporation.- The European Regulatory Environment of RNA-Based Vaccines.- Discovery and Subtyping of Neo-Epitope Specific T-cell Responses for Cancer Immunotherapy: Addressing the Mutanome.- Considerations for Producing mRNA Vaccines for Clinical Trials.- Nonclinical Safety Testing of RNA Vaccines.- Immunotherapy of Uveal Melanoma: Vaccination Against Cancer.

Erscheinungsdatum	13.01.2017
Reihe/Serie	Methods in Molecular Biology ; 1499
Zusatzinfo	26 Illustrations, color; 16 Illustrations, black and white; XI, 282 p. 42 illus., 26 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
Schlagworte	non-replicating mRNA vectors • recombinant viruses • self-amplifying mRNA • vector platforms • Viruses
ISBN-10	1-4939-6479-8 / 1493964798
ISBN-13	978-1-4939-6479-6 / 9781493964796
Zustand	Neuware