An in vitro study of the expression, purification and intramolecular interactions of Hepatoma Up Regulated Protein (HURP)

Eva Ka (Autor)

Buch | Softcover

108 Seiten

2016 | 16001 A. 1. Auflage
GRIN Verlag
978-3-668-19043-6 (ISBN)

Infos

Bachelor Thesis from the year 2011 in the subject Biology - Micro- and Molecular Biology, grade: A+ or 10.0, Democritus University Of Thrace (Molecular Biology and Genetics), language: Modern Greek, abstract: This manuscript is a diploma undergraduate thesis written in Greek; Research was conducted at the University of Thrace, at the Department of Molecular Biology and Genetics. Hepatoma Up Regulated Protein (HURP) is a microtubule-associated protein essential for the assembly of the mitotic spindle. HURP binds microtubules via its N-terminal domain, which is inhibited by HURP's C-terminal tail. The kinase Aurora A activates HURP by phosphorylating its C-terminus, which dissociates from the N-terminal microtubule binding domain.This diploma thesis explores how the autoinhibitory interaction between the N- and C-termini of HURP affect binding to proteins with key functions in spindle assembly (e.g., the mitotic motor Eg5 and the microtubule associated proteins TPX2 andXMAP125). I used molecular cloning techniques to generate recombinant GST- and His tagged chimeras of HURP's N- and C-termini. I optimized conditions for the expression of these proteins in bacteria and their purification by liquid chromatography methods using an FPLC system. I performed pull-down assays by incubating the recombinant proteins with HeLa cell extracts and ran western blots to examine the interactions of HURP with Eg5, TPX2 and XMAP125.

Produkt-Details

• Erscheinungsdatum: 06.12.2020
• Sprache: Greek, Modern (1453-)
• Maße: 148 x 210 mm
• Gewicht: 167 g
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete
• Themenwelt: Medizin / Pharmazie ► Studium
• Themenwelt: Naturwissenschaften ► Biologie ► Allgemeines / Lexika
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Schlagworte: Mikrobiologie (nicht-medizinisch)
• ISBN-10: 3-668-19043-7 / 3668190437
• ISBN-13: 978-3-668-19043-6 / 9783668190436
• Zustand: Neuware