Methods in Skin Research
John Wiley & Sons Ltd (Verlag)
978-0-471-10512-1 (ISBN)
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Research into the structure, function and pathology of skin is carried out by clinicians and scientists from an unusually wide range of disciplines. The reasons for this include the size and accessibility of the skin and the wide range of pathological conditions to which it is subject. A result of this diversity of interest, is that the literature on skin research is widely distributed amongst the biological, medical and physical science journals. Meetings are often similarly wide in scope and it is extremely difficult for a worker to understand in any detail more than a small proportion of the work presented. In this respect standard textbooks on, for example, biochemistry are of limited application as many classical techniques have to be adapted before use on such a tough and insoluble tissue as the skin.
This book aims firstly, to promote critical awareness in the field by having a variety of techniques described in detail and in the context of their application to skin research; secondly, to provide scientists and dermatologists wishing to use a technique for the first time with the information which they require in a readily accessible form, and thirdly, to counteract the inappropriate use of techniques by discussing their relative advantages and disadvantages in depth. Particular features are the inclusion of each author's unique "tricks of the trade", discussion of the amount of training needed by a prospective user and the time required to perform experiments. In each case a bibliography of other descriptions and applications is given, together with any appropriate experimental minutiae such as the composition of buffers, names of suppliers etc. .pa
Preface; Transmission Electron Microscopy; The Freeze-Fracture Replication Technique in Studies of the Skin; Autoradiography; Antigenic Markers of Epidermal Langerhans Cells: Light and Electron Microscopic Immuno-cytochemical Methods of Detection; In Vitro Methods for Studying Lymphocyte Functions; Detection of Circulating Immune Complexes; Cultivation of Normal Human Epidermal Keratinocytes and Melanocytes; Methods for the Use of Epidermal Cell Culture to Study Chemical Carcinogenesis; Human Skin-Graft-Nude Mouse Model: Techniques and Application; Epidermal Cell Population Kinetics; Hormonal Actions on Hair Follicles and Associated Glands; Hair; Analysis of Sebaceous Lipids; Methods in Epidermal Lipid Research; Skin Permeability; Microbiology of Skin; Methods in Photobiological Research; Assay of Cyclic Nucleotides in Epidermis; Inflammatory Mediators; Dermal Vasculature; Collagen in Skin: Preparation and Analysis; Techniques for Studying Epidermal Glycosaminoglycan Synthesis; A Survey of Methods for the Isolation and Fractionation of Epidermal Tissue and Cells; Index.
Erscheint lt. Verlag | 20.2.1985 |
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Zusatzinfo | illustrations, bibliography, index |
Verlagsort | Chichester |
Sprache | englisch |
Maße | 150 x 230 mm |
Gewicht | 1487 g |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Dermatologie |
ISBN-10 | 0-471-10512-0 / 0471105120 |
ISBN-13 | 978-0-471-10512-1 / 9780471105121 |
Zustand | Neuware |
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