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Marine Genomics

Methods and Protocols

Sarah J. Bourlat (Herausgeber)

Buch | Hardcover
253 Seiten
2016 | 1st ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-3772-1 (ISBN)

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This volume presents the latest protocols for both laboratory and
bioinformatics based analyses in the field of marine genomics. The chapters
presented in the book cover a wide range of topics, including the sampling and
genomics of bacterial communities, DNA extraction in marine organisms,
high-throughput sequencing of whole mitochondrial genomes, phylogenomics, SNP
discovery, SNP-arrays for species identification, digital PCR-based
quantification methods, environment DNA for invasive species surveillance and
monitoring, microarrays for the detection of waterborne pathogens, DNA
barcoding of marine biodiversity, metabarcoding protocols for marine
eukaryotes, analytical protocols for the visualization of eukaryotic diversity,
and applications for genomic data to benthic indices for environmental
monitoring. Written in the highly successful Methods in Molecular Biology
series format, chapters include introduction to their respective topics, lists
of the necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Marine Genomics: Methods and Protocols is a
valuable resource for researchers, students, and policy makers in the field of
marine biology.

Sampling
of Riverine or Marine Bacterial Communities in Remote Locations: From Field to Publication.- DNA Extraction Protocols for Whole Genome Sequencing
in Marine Organisms.- High-Throughput
Sequencing of Complete Mitochondrial Genomes.- Phylogenomics
Using Transcriptome Data.- SNP
Discovery Using Next Generation Transcriptomic Sequencing.- SNP-Arrays for Species Identification in
Salmonids.- The
Next Generation PCR-Based Quantification Method for Ambient Waters: Digital PCR.- Using Environmental DNA for Invasive Species
Surveillance and Monitoring.- Microarrays/DNA Chips for the Detection
of Waterborne Pathogens.- DNA Barcoding of Marine Metazoans.- DNA Barcoding Marine Biodiversity: Steps
from Mere Cataloguing to Giving Reasons for Biological Differences.- Metabarcoding Marine Sediments: Preparation of Amplicon Libraries.- Preparation of Amplicon Libraries for Metabarcoding
of Marine Eukaryotes Using Illumina MiSeq: The Dual-PCRMethod.- Preparation of Amplicon Libraries for Metabarcoding
of Marine Eukaryotes Using Illumina MiSeq: The Adaptor-Ligation Method.- Visualizing Patterns of Marine Eukaryotic
Diversity from Metabarcoding Data Using QIIME.- Analysis
of Illumina MiSeq Metabarcoding Data: Application to Benthic Indices for Environmental
Monitoring.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1452
Zusatzinfo 24 Illustrations, color; 22 Illustrations, black and white; X, 253 p. 46 illus., 24 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete
Studium 2. Studienabschnitt (Klinik) Humangenetik
Naturwissenschaften Biologie Botanik
Naturwissenschaften Biologie Zoologie
Schlagworte bacterial communities • DNA extraction in marine organisms • High-throughput sequencing • invasive species surveillance • metabarcoding eukaryotic • mitochondrial genomes • Phylogenomics • SNP discovery
ISBN-10 1-4939-3772-3 / 1493937723
ISBN-13 978-1-4939-3772-1 / 9781493937721
Zustand Neuware
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