Dendritic Cell Protocols
Humana Press Inc. (Verlag)
978-1-4939-3604-5 (ISBN)
The third edition of this
volume is aimed at providing both beginners and more experienced researchers a
choice of methods to isolate and analyze dendritic cells(DC). An introductory
review provides an overview of recent advances in the characterization of DC
subsets in mouse and human. While additional chapters provide methods to
culture human and mouse dendritic cells, protocols for the isolation of
dendritic cells, the isolation of dendritic cell progenitors from mouse, and
the purification of dendritic cells from human blood. Written in the highly
successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Authoritative and
cutting-edge, Dendritic Cell
Protocols, Third Edition aims to ensure successful results in the further
study of this vital field.
Review of Mouse and Human Dendritic Cell Subsets.- In
Vitro Generation of Human Xcr1+ Dendritic Cells from Cd34+
Hematopoietic Progenitors.- Derivation
and Utilization Of Functional Cd8+ Dendritic Cell Lines.- Isolation
of Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from Mouse.- The
Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen
Dendritic Cell Populations and their In
Vitro Equivalents.- Dendritic
Cell Subsets Purification from Human Tonsils and Lymph Nodes.- Isolation
and Identification of Conventional Dendritic Cell Subsets from the Intestine of
Mice and Men.- Isolation
of Human Skin Dendritic Cell Subsets.- Isolation of Mouse Dendritic Cell Subsets and
Macrophages from Skin.- Isolation of Conventional Dendritic Cells from
Mouse Lungs.- Purification
of Human Dendritic Cell Subsets from Peripheral Blood.- Protocols for the Identification andIsolation of
Antigen-Presenting Cells in Human and Mouse Tissues.- Measurement of Export to the Cytosol in Dendritic
Cells Using a Cytofluorimetry-Based Assay.- Cross-Presentation Assay for Human Dendritic Cells.- Analysis of
Intracellular Trafficking of Dendritic Cell Receptors for Antigen Targeting.- Characterization
of Dendritic Cell Subsets through Gene Expression Analysis.- In Vivo Ablation of a Dendritic Cell Subset
Expressing a Chemokine Receptor, Xcr1.- In Vivo Analysis Of Intestinal
Mononuclear Phagocytes.- In Vivo Imaging Of Cutaneous Dcs In Mice.- Analysis of Dendritic Cell Function by Clec9a-Dtr
Transgenic Mice.- Analysis
of Dc Functions Using Cd205-Dtr Knock-In Mice.- Generation of Humanized Mice for Analysis of
Human Dendritic Cells.
| Erscheinungsdatum | 08.10.2016 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1423 |
| Zusatzinfo | 27 Illustrations, color; 41 Illustrations, black and white; XI, 322 p. 68 illus., 27 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Studium ► 1. Studienabschnitt (Vorklinik) ► Anatomie / Neuroanatomie |
| Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Schlagworte | culture human dendritic cells • culture mouse dendritic cells • DC subsets • dendritic cell progenitors • human blood |
| ISBN-10 | 1-4939-3604-2 / 1493936042 |
| ISBN-13 | 978-1-4939-3604-5 / 9781493936045 |
| Zustand | Neuware |
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