Gene Transfer in the Cardiovascular System (eBook)

Experimental Approaches and Therapeutic Implications

Keith L. March (Herausgeber)

eBook Download: PDF

2012
Springer US (Verlag)
978-1-4615-6277-1 (ISBN)

Infos

The goal of gene transfer is protein expression. a process brought about by the insertion of a gene coding for a foreign protein into target cells resulting in the synthesis of the foreign protein For gene therapy, a tmnsferred therapeutic gene must be expressed at a level beneficial for the patient. This chapter provides an introductory overview of the rapidly evolving field of non-viral approaches for gene delivery to rnarnrnalian cells. Although currently there are fewer ongoing clinical trials using non-viral approaches than those using viral based systems, the number of non-viral trials is increasing. The long range goal of some research groups is the development of a genetically engineered artificial virus targeted to specific cells in the human body. An arurual conference, organized by Cambridge Healthtech Institute entitled "e;Artificial Self-Assembling Systems for Gene Transfer"e;, brings together researchers interested in this field [1]. Assembly of an artificial virus is very complex; other research groups aim to develop simpler delivery systems consisting of a plasmid combined with delivery agents. Viral-based systems are very successful for gene delivery, but despite their successes, viral-based systems have some geneml limitations and system-specific limitations. When employing a viml-based system, the following limitations should be considered: * size limitation of the inserted gene due to packaging constraints (e. g. adenovirus, retrovirus) . * potential tumorigenesis (e. g. retrovirus) * potential for insertional mutagenesis (greater than plasmid based systems) * potential imrnunogenicity (e. g.

Produkt-Details

• Erscheint lt. Verlag: 6.12.2012
• Sprache: englisch
• Themenwelt: Medizinische Fachgebiete ► Innere Medizin ► Kardiologie / Angiologie
• Themenwelt: Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
• ISBN-10: 1-4615-6277-5 / 1461562775
• ISBN-13: 978-1-4615-6277-1 / 9781461562771